Human CSF-1: molecular cloning and expression of 4-kb cDNA encoding the human urinary protein

Journal Article · Fri Mar 20 04:00:00 EST 1987 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.3493529· OSTI ID:7000228

Wong, G G; Temple, P A; Leary, A C; Witek-Giannotti, J S; Yang, Y; Ciarletta, A B; Chung, M; Murtha, P; Kriz, R; Kaufman, R J; Ferenz, C R

A 4-kilobase complementary DNA (cDNA) encoding human macrophage-specific colony-stimulating factor (CSF-1) was isolated. When introduced into mammalian cells, this cDNA directs the expression of CSF-1 that is structurally and functionally indistinguishable from the natural human urinary CSF-1. Direct structural analysis of both the recombinant CSF-1 and the purified human urinary protein revealed that these species contain a sequence of at least 40 amino acids at their carboxyl termini which are not found in the coding region of a 1.6-kilobase CSF-1 cDNA that was previously described. These results demonstrate that the human CSF-1 gene can be expressed to yield at least two different messenger RNA species that encode distinct but related forms of CSF-1.

• Research Organization: Genetics Institute, Inc., Cambridge, MA (USA)
• OSTI ID: 7000228
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 235; ISSN SCIEA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CELL PROLIFERATION
CLONING
DNA
DNA-CLONING
GENE REGULATION
GROWTH FACTORS
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
MITOGENS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RECOMBINANT DNA
RNA
URINE
VERTEBRATES
WASTES