Characterization of alveolar macrophage receptors involved in opsonin independent phagocytosis
Hamster alveolar macrophages (AM) avidly ingest particles in the absence of serum. This process is calcium dependent and can be blocked by HAMM, a mouse monoclonal antibody specific for hamster AM's. The purpose of this study was to identify the membrane receptors involved. AM membranes were labeled with /sup 125/I, lysed with RIPA-PMSF, and then reacted with uncoated latex beads or with beads coated with BSA, gelatin, or poly-L-lysine. Lysed membranes were also reacted with zymosan particles. All of these reactions were done in the presence and absence of calcium and magnesium ions. After reaction, the particles were boiled in SDS to remove attached membrane constituents which were then separated by polyacrylamide gel electrophoresis. Only one AM membrane protein (37 kilodaltons (kd)) bound to BSA and gelatin coated latex, uncoated latex, and zymosan particles in the presence of Ca/sup + +/ and Mg/sup + +/. Proteins of 45 and 19 kd attached to all particles even in the absence of divalent cations. In contrast, HAMM reacted with a membrane constituent of 102 kd. The authors conclude that the Ca/sup + +/ dependent receptor for opsonin independent phagocytosis has a molecular weight of 37 kd and is different from the antigen identified by HAMM.
- Research Organization:
- Harvard School of Public Health, Boston, MA
- OSTI ID:
- 6999959
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
CALCIUM COMPOUNDS
CELL CONSTITUENTS
CELL MEMBRANES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
HAMSTERS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MACROPHAGES
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
MOLECULAR WEIGHT
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHAGOCYTES
PHAGOCYTOSIS
PROTEINS
PURIFICATION
RADIOISOTOPES
RECEPTORS
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES