Nutritionally induced quiescence of 9L gliosarcoma cells: correlation of cell cycle kinetics and radioresistance
Thesis/Dissertation
·
OSTI ID:6999537
Experiments were performed to compare the X-ray response of unfed plateau phase 9L gliosarcoma cells with exponentially growing, and synchronized 9L cells in the G/sub 1/ phase of the cell cycle. An insight may be gained as to why unfed plateau-phase 9L brain tumor cells are radioresistant. The onset of quiescence in the plateau phase as the 9L cultures age was the result of nutrient deprivation and not a cell contact effect. The quiescent population had a >90% G/sub 1/ DNA content. When the cultures were plated immediately after X-irradiation the quiescent cells were more radioresistant than the exponentially growing cells. The radioresistance was characterized by a larger shoulder on the survival curve of the quiescent cells, which indicated that more repair had occurred in the quiescent cells.
- Research Organization:
- California Univ., Berkeley (USA)
- OSTI ID:
- 6999537
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
BRAIN
CELL CYCLE
CENTRAL NERVOUS SYSTEM
DNA REPAIR
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IONIZING RADIATIONS
NERVOUS SYSTEM
NUTRITION
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
SURVIVAL CURVES
TUMOR CELLS
X RADIATION
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
BRAIN
CELL CYCLE
CENTRAL NERVOUS SYSTEM
DNA REPAIR
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IONIZING RADIATIONS
NERVOUS SYSTEM
NUTRITION
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
SURVIVAL CURVES
TUMOR CELLS
X RADIATION