Investigation of the effect of radiation of DNA methylation patterns

Thesis/Dissertation · Wed Jan 01 04:00:00 EST 1986

OSTI ID:6999519

Kalinich, J F

Four lines of cultured mammalian cells were used in this project: V79A03, a Chinese hamster lung fibroblast; HeLa S-3, an epithelial cell line from a human cervix carcinoma; CHO K-1, an epithelial cell line from a Chinese hamster ovary; and C-1300 N1E-115, a mouse neuroblastoma line. The 5-methylcytosine levels in DNA following exposure to cobalt-60 gamma radiation were measured. Induction of metallothionein in V79A03 cells and acetylcholinesterase in C-1300 N1E-115 cells after irradiation was determined and the effect of radiation on cytoplasmic and nuclear levels of DNA methyltransferase was studied and nuclear demethylase activity assayed. This study showed gamma radiation resulted in a decrease of 5-methylcytosine levels in the DNA of cultured mammalian cells. This radiation-induced hypomethylation resulted in the induction of acetylcholinesterase in mouse neuroblastoma cells and metallothionein in Chinese hamster lung fibroblasts and was caused by a decrease in DNA methyltransferase activity in the nucleus after irradiation and not by the presence of DNA demethylase.

• Research Organization: American Univ., Washington, DC
• OSTI ID: 6999519
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLCHOLINE
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBOXYLESTERASES
CHEMICAL REACTIONS
CHOLINESTERASE
CONNECTIVE TISSUE CELLS
DNA
DRUGS
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
EPITHELIUM
ESTERASES
ESTERS
FEMALE GENITALS
FIBROBLASTS
GAMMA RADIATION
GONADS
HAMSTERS
HYDROLASES
IONIZING RADIATIONS
LUNGS
MAMMALS
METALLOPROTEINS
METALLOTHIONEIN
METHYLATION
MICE
NEUROREGULATORS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OVARIES
PARASYMPATHOMIMETICS
PROTEINS
QUATERNARY COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
TISSUES
TRANSFERASES
TUMOR CELLS
VERTEBRATES