6(5)Carboxyfluorescein as a tracer of phloem sap translocation
- Ecole Nationale Superieure Agronomique, Montpellier (France)
- USTL, Montpellier (France)
6(5)carboxyfluorescein (6(5)CF), a polar fluorescein with an apparent pK of 6.3, was introduced, as a pH 6.3 solution, into the apoplast of lamina or petioles of mature soybean leaves. Freehand sections were prepared at various times and immediately observed with a fluorescence microscope. 6(5)CF-associated fluorescence appeared in all sink organs, from shoot apex to roots. It was strictly confined to the phloem regions, even after 4 days. Its transport into young leaves ceased at approximately the time they underwent sink-to-source transition. It was never transported between two leaflets of the same leaf. Its transport was interrupted by phloem destruction. All these transport characteristics were highly reproducible, and were paralleled by those of {sup 14}C transport after application of ({sup 14}C) sucrose to leaf surfaces. In contrast with 6(5)CF, fluorescein was transported between mature leaves, and between leaflets of the same, leaf. It was not restricted to phloem, and often appeared in the xylem region. These results indicate that 6(5)CF can be used to monitor phloem sap translocation in real time, in short- and long-term experiments.
- OSTI ID:
- 6998899
- Journal Information:
- American Journal of Botany; (USA), Journal Name: American Journal of Botany; (USA) Vol. 76:6; ISSN 0002-9122; ISSN AJBOA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AROMATICS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
DISACCHARIDES
DYES
FLUORESCEIN
FLUORESCENCE
GLYCINE HISPIDA
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEAVES
LEGUMINOSAE
LUMINESCENCE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
OLIGOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHENOLS
PHYSIOLOGY
PLANTS
POLYPHENOLS
SACCHARIDES
SACCHAROSE
TRACER TECHNIQUES