Enhancement of enzymatic hydrolysis of cellulose by surfactant

Journal Article · Wed Jan 01 00:00:00 EST 1986 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260281117· OSTI ID:6998591

Ooshima, H; Sakata, M; Harano, Y

Effects of surfactants on enzymatic saccharification of cellulose have been studied. Nonionic, amphoteric, and cationic surfactants enhanced the saccharification, while anionic surfactant did not. Cationic and anionic surfactants denatured cellulase in their relatively low concentrations, namely, more than 0.008 and 0.001%, respectively. Using nonionic surfactant Tween 20, which is most effective to the enhancement (e.g., the fractional conversion attained by 72 h saccharification of 5 wt % Avicel in the presence of 0.05 wt % Tween 20 is increased by 35%), actions of surfactant have been examined. As the results, it was suggested that Tween 20 plays an important role in the hydrolysis of crystalline cellulose and that Tween 20 disturbs the adsorption of endoglucanase on cellulose, i.e., varies the adsorption balance of endo- and exoglucanase, resulting in enhancing the reaction. The influence of Tween 20 to the saccharification was found to remain in simultaneous saccharification and fermentation of Avicel.

• Research Organization: Osaka City Univ., Japan
• OSTI ID: 6998591
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 28:11
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
CELLULOSE
ENZYMATIC HYDROLYSIS
SACCHARIFICATION
SURFACTANTS
CHEMICAL REACTIONS
TRICHODERMA VIRIDE
ENZYME ACTIVITY
GLUCOSE
ALDEHYDES
CARBOHYDRATES
DECOMPOSITION
FUNGI
HEXOSES
HYDROLYSIS
LYSIS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
TRICHODERMA
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)