Enhanced detection of fluorescence quenching in labeled cells
This patent describes a method for relatively quantifying BrdU-labeled DNA in cells in an S-phase during a selected interval within a cell cycle. It comprises: incorporating the BrdU into the DNA for a time of about 5 minutes to thirty minutes; staining the DNA with a first fluorochrome having a fluorescence which is quenchable by BrdU; staining the DNA with a second fluorochrome having a fluorescence which is substantially unaffected by BrdU; measuring fluorescence from the first and second fluorochromes to obtain first and second output signals, respectively, on a cell-by-cell basis; and subtracting the first output signal from the second output signal to obtain a different signal functionally related to the quantity of incorporated BrdU.
- Assignee:
- Dept. of Energy, Washington, DC (United States)
- Patent Number(s):
- US 5084378; A
- Application Number:
- PPN: US 7-126155
- OSTI ID:
- 6998285
- Resource Relation:
- Patent File Date: 30 Nov 1987
- Country of Publication:
- United States
- Language:
- English
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