Enhanced detection of fluorescence quenching in labeled cells

Patent · Tue Jan 28 04:00:00 EST 1992

OSTI ID:6998285

Crissman, H A; Steinkamp, J A

This patent describes a method for relatively quantifying BrdU-labeled DNA in cells in an S-phase during a selected interval within a cell cycle. It comprises: incorporating the BrdU into the DNA for a time of about 5 minutes to thirty minutes; staining the DNA with a first fluorochrome having a fluorescence which is quenchable by BrdU; staining the DNA with a second fluorochrome having a fluorescence which is substantially unaffected by BrdU; measuring fluorescence from the first and second fluorochromes to obtain first and second output signals, respectively, on a cell-by-cell basis; and subtracting the first output signal from the second output signal to obtain a different signal functionally related to the quantity of incorporated BrdU.

• Assignee: Dept. of Energy, Washington, DC (United States)
• Patent Number(s): A; US 5084378
• Application Number: PPN: US 7-126155
• OSTI ID: 6998285
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400101 -- Activation
Nuclear Reaction
Radiometric & Radiochemical Procedures
400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550201 -- Biochemistry-- Tracer Techniques
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
CELL CYCLE
DNA
EMISSION SPECTROSCOPY
FLUORESCENCE SPECTROSCOPY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
NUCLEIC ACIDS
ORGANIC COMPOUNDS
SPECTROSCOPY
TRACER TECHNIQUES