5'Azido-N-1-napthylphthalamic acid, a photolabile analog of N-1-naphthylphthalamic acid

Journal Article · Tue Sep 01 04:00:00 EDT 1987 · Plant Physiol.; (United States)

OSTI ID:6998110

Voet, J G; Howley, K S; Shumsky, J S

A photolabile analog of N-1-naphthylphthalamic acid (NPA), 5'-azido-N-1-naphthylphthalamic acid (Az-NPA), has been synthesized and characterized. This potential photoaffinity label for the plasma membrane NPA binding protein competes with (/sup 3/H)NPA for binding sites on Curcurbita pepo L. (zucchini) hypocotyl cell membranes with K/sub 0.5/ = 2.8 x 10/sup -7/ molar. The K/sub 0.5/ for NPA under these conditions is 2 x 10/sup -8/ molar, indicating that the affinity of Az-NPA for the membranes is only 14-fold lower than NPA. While the binding of Az-NPA to NPA binding sites is reversible in the dark, exposure of the Az-NPA treated membranes to light results in a 30% loss in (/sup 3/H)NPA binding ability. Pretreatment of the membranes with NPA protects the membranes against photodestruction of (/sup 3/H)NPA binding sites by Az-NPA supporting the conclusion that Az-NPA destroys these sites by specific covalent attachment.

• Research Organization: Swarthmore College, PA (USA)
• OSTI ID: 6998110
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 85:1; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CELL MEMBRANES
FOOD
HERBICIDES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MEMBRANES
ORGANIC COMPOUNDS
PESTICIDES
PHOTOSENSITIVITY
PLANT CELLS
PLANTS
PROTEINS
REACTION KINETICS
SENSITIVITY
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VEGETABLES