Compartmental efflux analysis and removal of extracellular cadmium from roots. [Agrostis gigantea]
Profiles of /sup 109/Cd efflux from roots into three solutions were determined for young intact plants of Agrostis gigantea and maize. The solutions were (a) nutrient culture medium containing 3 micromolar Cd at room temperature, (b) ice-cold 5 millimolar CaCl/sub 2/, and (c) ice-cold 5 millimolar PbCl/sub 2/. Efflux profiles were clearly resolved into three easily discernible components having fast, medium, and slow exchange rates. These results were unexpected for the situation where some intracellular Cd was present both as extractable Cd-binding peptide and in electron-dense granules within the cytoplasm and the vacuoles. Adding a fourth compartment to the curve-fitting model produced a splitting of the fast exchanging component. Use of these efflux kinetics to estimate Cd fluxes through membranes was inappropriate. However, they were useful in determining optimal washing times for the removal of extracellular Cd. A 10 minute wash in ice-cold 5 millimolar CaCl/sub 2/ is recommended for this purpose for Agrostis and maize roots.
- Research Organization:
- Univ. of Guelph, Ontario (Canada)
- OSTI ID:
- 6998109
- Journal Information:
- Plant Physiol.; (United States), Vol. 85:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CADMIUM
BIOCHEMICAL REACTION KINETICS
REMOVAL
ROOTS
EXTRACELLULAR SPACE
CADMIUM 109
CALCIUM CHLORIDES
CELL FLOW SYSTEMS
CYTOPLASM
LABELLED COMPOUNDS
LEAD CHLORIDES
MAIZE
MEMBRANE TRANSPORT
PEPTIDES
PLANT CELLS
TRACER TECHNIQUES
WASHING
ALKALINE EARTH METAL COMPOUNDS
BETA DECAY RADIOISOTOPES
CADMIUM ISOTOPES
CALCIUM COMPOUNDS
CALCIUM HALIDES
CELL CONSTITUENTS
CEREALS
CHLORIDES
CHLORINE COMPOUNDS
CLEANING
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
GRASS
HALIDES
HALOGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEAD COMPOUNDS
LEAD HALIDES
METALS
NUCLEI
ORGANIC COMPOUNDS
PLANTS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SPACE
YEARS LIVING RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques