Regulation and function of the alpha/sub 2/ adrenergic autoreceptor in the central nervous system
The purpose of this investigation was to determine whether changes observed in the number of alpha/sub 2/ adrenergic receptors in the brain as measured by radioligand binding experiments reflect changes in the function of alpha/sub 2/ autoregulatory receptors which are located on noradrenergic nerve terminals. Inhibition by clonidine of field stimulated /sup 3/H-norepinephrine (/sup 3/H-NE) release from rat hippocampal slices before and after several drug treatments was analyzed to investigate changes in alpha/sub 2/ adrenergic receptor function. Clonidine in a concentration-dependent manner inhibited /sup 3/H-NE release. The effect of clonidine was blocked by the specific alpha/sub 2/ adrenergic receptor antagonist, idazoxan. The cumulative administration of clonidine generated a smooth and well-fitted log-concentration-effect curve. Results are presented which demonstrate that this technique can be employed to investigate the role of changes in the function of the alpha/sub 2/ autoregulatory receptor. The present investigation also examined representatives of four drug classes which have been shown to alter the specific binding of /sup 3/H-clonidine to neural membranes to determine whether changes in the alpha/sub 2/ autoregulatory receptor function also occur.
- Research Organization:
- Michigan Univ., Ann Arbor (USA)
- OSTI ID:
- 6998062
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
NORADRENALINE
BIOSYNTHESIS
RECEPTORS
BIOLOGICAL FUNCTIONS
SYMPATHOMIMETICS
BRAIN
CENTRAL NERVOUS SYSTEM
INHIBITION
LIGANDS
RATS
SYMPATHOLYTICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ADRENAL HORMONES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
DRUGS
FUNCTIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RODENTS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques