Studies on the neuronal Golgi apparatus-complex. I. Quantitative ultrastructural autoradiographic analysis of the endocytosis into the Golgi complex of wheat germ agglutinin by cultured murine neuroblastoma cells. II. Identification of a 160 Kd polypeptide of the medial cisterns of the Golgi complex

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:6998027

Mezitis, S G.E.

An ultrastructural autoradiographic method is presented which quantitates the sequential endocytosis of tritiated wheat germ agglutinin (N-(acetyl-/sup 3/H)-WGA) into the Golgi complex and lysosomes of cultured murine neuroblastoma cells. Cells were incubated with /sup 3/H-WGA for one hour at 4/sup 0/C, washed and incubated in complete medium without ligand at 37/sup 0/C for 5, 15, 30, and 120 minutes. At 15 minutes, the optimized sources/..mu..m/sup 2/ of neuroblastoma cell area, which represented the grain density of each compartment, were: Golgi associated vesicles, i.e. clusters of vesicles within a one micron radius of the Golgi cisterns, (1.41 + -0.28), Golgi cisterns (0.73 + -0.41), lysosomes (0.1 + -0.09); at two hours, Golgi associated vesicles exhibited some labeling ((0.71 + -0.1), while lysosomes were heavily labeled (2.17 + -0.22). These results are consistent with the hypotheses that either the Golgi complex (cisterns and associated vesicles) is an early and intermediate step of the endocytosis of /sup 3/H-WGA into lysosomes, or that it constitutes part of a separate and quantitatively significant pathway of endocytosis of this ligand.

• Research Organization: Pennsylvania Univ., Philadelphia (USA)
• OSTI ID: 6998027
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
MOLECULAR STRUCTURE
PHAGOCYTOSIS
AUTORADIOGRAPHY
CELL CONSTITUENTS
LYSOSOMES
MICE
NERVE CELLS
POLYPEPTIDES
TRITIUM COMPOUNDS
TUMOR CELLS
ANIMAL CELLS
ANIMALS
ANTIBODIES
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANOIDS
PEPTIDES
PROTEINS
RODENTS
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques