The bean. alpha. -amylase inhibitor is encoded by a lectin gene
- Univ. of California, San Diego, La Jolla (USA)
The common bean, Phaseolus vulgaris, contains an inhibitor of insect and mammalian {alpha}-amylases that does not inhibit plant {alpha}-amylase. This inhibitor functions as an anti-feedant or seed-defense protein. We purified this inhibitor by affinity chromatography and found that it consists of a series of glycoforms of two polypeptides (Mr 14,000-19,000). Partial amino acid sequencing was carried out, and the sequences obtained are identical with portions of the derived amino acid sequence of a lectin-like gene. This lectin gene encodes a polypeptide of MW 28,000, and the primary in vitro translation product identified by antibodies to the {alpha}-amylase inhibitor has the same size. Co- and posttranslational processing of this polypeptide results in glycosylated polypeptides of 14-19 kDa. Our interpretation of these results is that the bean lectins constitute a gene family that encodes diverse plant defense proteins, including phytohemagglutinin, arcelin and {alpha}-amylase inhibitor.
- OSTI ID:
- 6994782
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ENZYME INHIBITORS
GENES
PHASEOLUS
MOLECULAR BIOLOGY
AMINO ACID SEQUENCE
AMYLASE
LECTINS
POLYPEPTIDES
POST-TRANSLATION MODIFICATION
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MOLECULAR STRUCTURE
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PEPTIDES
PLANTS
PROTEINS
550201* - Biochemistry- Tracer Techniques