Human tumor antigens identified with monoclonal antibodies
Thesis/Dissertation
·
OSTI ID:6993801
MoAbLc1 (IgM) and MoAbLc2 (IgG/sub 2a/) were produced against human lung carcinoma cell line (ChaGo). Lc1 recognizes a approx. = 330-kd/approx. = 310-kd glycoprotein complexes, and Lc2 recognizes a approx. = 60-kd/approx. = 47-kd protein complex. With a panel of cell lines of different tissue origin, Lc1 showed a more restricted reactivity to ChaGo; it cross-reacted with another lung carcinoma cell line (SK-Lc-2) and two breast carcinoma cell lines, but failed to react with cell lines of fetal lung, of colon, esophageal, prostate, stomach, and ovarian carcinomas, of B and T lymphoblastoid cells, neuroblastomas, glioblastoma, astrocytoma, and human peripheral blood lymphocytes. New and improved methods were developed for the production of indium-111-labeled MoAbs for tumor imaging. To facilitate the application of bicyclic anhydride diethylenetriaminepentaacetic acid (BADTPA) to In-111 labeling of antibodies, we have modified the original method by using C-14-labeled BADTPA, which allows precise quantitation of DTPA molecules incorporated. A new heterobifunctional reagent, 2,6-dioxo-N-(carboxyl)morpholine (DCM) was synthesized for chelating In-111 to MoAbs, and demonstrated higher retention of immunoreactivity of the labeled antibody.
- Research Organization:
- Columbia Univ., New York (USA)
- OSTI ID:
- 6993801
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
CARBON 14 COMPOUNDS
CARCINOMAS
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC USES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
MAMMALS
MAN
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
PRIMATES
RADIOISOTOPES
TRACER TECHNIQUES
USES
VERTEBRATES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
CARBON 14 COMPOUNDS
CARCINOMAS
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC USES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
MAMMALS
MAN
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
PRIMATES
RADIOISOTOPES
TRACER TECHNIQUES
USES
VERTEBRATES