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Studies of double-labeled mouse thyrotropin and free alpha-subunits to estimate relative fucose content

Journal Article · · Proc. Soc. Exp. Biol. Med.; (United States)
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The composition and structure of the complex oligosaccharides of thyrotropin (TSH) and free alpha-subunits are not well established, but are believed to be important determinants of the biological properties of these glycoproteins. We employed a simple double-label technique to learn the relative fucose content of mouse thyrotropin and free alpha-subunits. Thyrotropic tumor minces were incubated simultaneously with (/sup 35/S)methionine and (/sup 3/H)fucose. Thyrotropin and free alpha-subunits were labeled with both isotopes, and the ratio of /sup 3/H//sup 35/S was higher in free alpha-subunits than in thyrotropin; free alpha-subunits were approximately fivefold richer in fucose than was thyrotropin. The /sup 3/H//sup 35/S ratio was not substantially altered in TSH or free alpha-subunits secreted after a brief incubation with 10(-7) M thyrotropin-releasing hormone. Species which incorporated (/sup 3/H)fucose were resistant to endoglycosidase H. Thus, mouse free alpha-subunits secreted by thyrotropic tumor are relatively rich in fucose. Double-isotope labeling using an amino acid and a sugar appears to be a useful technique for studies of the glycoprotein hormones.
Research Organization:
Univ. of Chicago, IL
OSTI ID:
6992719
Journal Information:
Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 2; ISSN PSEBA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CHEMICAL ANALYSIS
DAYS LIVING RADIOISOTOPES
DOUBLE LABELLING
EVEN-ODD NUCLEI
HEXOSES
HORMONES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
MAMMALS
MICE
MONOSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOISOTOPES
RODENTS
SACCHARIDES
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TSH
VERTEBRATES