Depression of contraction and the calcium transient in single cardiomyocytes with acute ethanol exposure

Conference · Wed Jan 01 04:00:00 EST 1992 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:6992331

Rozanski, D J; Delaville, F J; Thomas, A P ^[1]

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1. Thomas Jefferson Univ., Philadelphia, PA (United States)

The mechanism by which acute ethanol (ET) exposure causes reversible myocardial dysfunction is unknown. The purpose of this study was to examine the effects of ET exposure on contraction and cytosolic free Ca[sup 2+] ([Ca[sup 2+]][sub i]) in electrically-stimulated single rat ventricular myocytes loaded with the fluorescent Ca[sup 2+] indicator, fura-2. Whole cell fluorescence images were obtained at high speed with a frame transfer CCD camera and processed by a digital imaging system which allowed simultaneous measurement of cell shortening and [Ca[sup 2+]][sub i] changes. A concentration- and time-dependent decline in cell shortening was observed during a 20 min period of ET perfusion. At 3% ET, maximal shortening was reduced by 65%. Cell shortening was restored following washout of the ET. In parallel with the depression of contractility, the magnitude of the [Ca[sup 2+]][sub i] transient was also reduced. ET had no significant effect on basal [Ca[sup 2+]][sub i] at concentrations up to 3%. Kinetic parameters of the [Ca[sup 2+]][sub i] transient, including rates of rise and decay were also depressed, although the time to peak was unaffected by ET. These results indicate that ET interferes with Ca[sup 2+] fluxes in the ventricular myocyte in a manner that may contribute to the contractile impairment of cardiac function induced by acute ET exposure in vivo.

• OSTI ID: 6992331
• Report Number(s): CONF-9202109--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 6:1
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ALCOHOLS
ALKALINE EARTH METAL COMPOUNDS
BIOLOGICAL EFFECTS
BODY
CALCIUM COMPOUNDS
CARDIOVASCULAR SYSTEM
CONTRACTION
DOSE-RESPONSE RELATIONSHIPS
ETHANOL
HEART
HYDROXY COMPOUNDS
MEMBRANE TRANSPORT
ORGANIC COMPOUNDS
ORGANS
SENSITIVITY
TIME DEPENDENCE