Effect of starvation on induction of quinoline degradation for a subsurface bacterium in a continuous-flow column

Journal Article · Sat Aug 01 04:00:00 EDT 1992 · Applied and Environmental Microbiology; (United States)

OSTI ID:6991880

Truex, M J; Brockman, F J; Fredrickson, J K ^[1]; Johnstone, D L ^[2]

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1. Pacific Northwest Lab., Richland, WA (United States)
2. Washington State Univ., Pullman (United States)

Differences in the induction response and the initial two reactions of quinoline degradation between short-term and long-term starved cells of a subsurface Pseudomonas cepacia strain were examined by using continuous-flow columns. The ability of bacteria that are indigenous to oligotrophic environments to respond to a contaminant was assessed by using long-term starvation to induce a cell physiology that stimulates the in situ physiology of the bacteria. With quinoline concentrations of 39 and 155 [mu]M, long-term-starved cells converted quinoline to degradation products more efficiently than did short-term-starved cells. Quinoline concentrations of 155 [mu]M and, to a greater extent, 775 [mu]M had an inhibitory effect on induction in long-term-starved cells. However, only the length of the induction process was affected with these quinoline concentrations; degradation of quinoline at the steady state for long-term-starved cells was equal to or better than that for short-term-starved cells. The induction time for short-term-starved cells did not increase progressively with increasing quinoline concentration. Experiments with starved cells are important for the development of accurate predictive models of contaminant transport in the subsurface because starvation, which induces a cell physiology that stimulates the in situ physiology of many bacteria, may affect the induction process.

• DOE Contract Number: AC06-76RL01830; FG06-89ER75522
• OSTI ID: 6991880
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 58:8; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540220 -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
AZAARENES
AZINES
BACTERIA
BIODEGRADATION
CHEMICAL REACTIONS
CLEANING
CONTINUOUS CULTURE
DECOMPOSITION
DECONTAMINATION
FASTING
GROWTH
HETEROCYCLIC COMPOUNDS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PSEUDOMONAS
PYRIDINES
QUINOLINES
REMEDIAL ACTION
SOILS