Structure of plant cell walls. XVIII. An analysis of the extracellular polysaccharides of suspension-cultured sycamore cells. [Acer pseudoplatanus]

Journal Article · Mon Mar 31 23:00:00 EST 1986 · Plant Physiol.; (United States)

OSTI ID:6991584

Stevenson, T T; McNeil, M; Darvill, A G; Albersheim, P

The water-soluble polysaccharides (SEPS) secreted into the medium by suspension-cultured sycamore cells were examined to determine whether the polysaccharides were the same as those present in the walls of sycamore cells. The SEPS were made more amenable to fractionation by treatment with a highly purified ..cap alpha..-1,4-endopolygalacturonase (EPG). The EPG-treated SEPS were fractionated by anion-exchange and gel-permeation chromatography. The following polysaccharides were found: xyloglucan, arabinoxylan, at least two arabinogalactans, a rhamnogalacturonan-II-like polysaccharide, and a polygalacturonic acid-rich polysaccharide. The oligogalacturonide fragments expected from EPG-digested homogalacturonan were also identified. Evidence was obtained for the presence of a rhamnogalacturonan-I-like polysaccharide. All of the above polysaccharides have been isolated from or are believed to be present in sycamore cell walls. Furthermore, all of the noncellulosic polysaccharides known to be present in sycamore cell-walls appears to be present in the SEPS.

• Research Organization: Univ. of Colorado, Boulder
• DOE Contract Number: AC02-76ER01426
• OSTI ID: 6991584
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 80:4; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMISTRY
CARBOHYDRATES
CELL CONSTITUENTS
CELL CULTURES
CELL WALL
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
ENZYMATIC HYDROLYSIS
FRACTIONATION
HYDROLYSIS
LYSIS
ORGANIC COMPOUNDS
PLANT CELLS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
STRUCTURAL CHEMICAL ANALYSIS
SYCAMORES
TREES