Quantitative role of the splanchnic bed in whole body leucine metabolism

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6991144

Yu, Y M; Wagner, D A; Tredget, E; Walesreweski, J; Burke, J F; Young, V R

The role of the splanchnic bed in the economy of whole body leucine (leu) metabolism was assessed in 5 chronically catheterized conscious fasting mongrel dogs. Using primed continuous intravenous infusions of L-(/sup 15/N, 1-/sup 13/C)-leu and L-1-/sup 14/C-leu the metabolic fate of leu carbon (C) and nitrogen (N) in the splanchnic region was compared with that in the body as a whole, by measurement of isotope and substrate balance across gut and liver. Sampling was from the portal and hepatic veins and arch of aorta. Blood flow estimation was made by dye dilution. Whole body leu N and C fluxes and oxidation were (Mean +/- SEM); 453 +/ 47, 197 +/- 37 and 41 +/- 5 ..mu..mol kg-1.h-1, respectively. The splanchnic bed accounted for (% of whole body) 36 +/- 13 of leu disappearance into proteins (liver 14%; gut 22%); 24 +/- 7 of leu appearance via protein breakdown (liver 8%; gut 16%) 12 +/- 2% of leu transamination to ..cap alpha..-ketoisocaproate (KIC) (liver 7%; gut 5%); 12 +/- 3 of KIC reamination to leu (liver 7%; gut 5%) and 11 +/- 3 of leu oxidation (liver 2%; gut 9%). Hence, in the fasting state the splanchnic region accounts for a small proportion of whole body leu-KIC interconversion and oxidation, but a more significant proportion of whole body of leu for protein synthesis.

• Research Organization: Massachusetts General Hospital, Boston
• OSTI ID: 6991144
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BLOOD FLOW
BODY
CARBON 13
CARBON 14
CARBON ISOTOPES
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
DOGS
EVEN-EVEN NUCLEI
EVEN-ODD NUCLEI
FUNCTIONS
GLANDS
ISOTOPE APPLICATIONS
ISOTOPES
LEUCINE
LIGHT NUCLEI
LIVER
MAMMALS
METABOLISM
NITROGEN 15
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
STABLE ISOTOPES
TRACER TECHNIQUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES