Defined bacterial culture development for methane generation from lactose. [Streptococcus lactis; Clostridium formicoaceticum; Methanococcus mazei]
The defined microbial cultures for methane generation from lactose were investigated. A mixed culture consisting of homolactic (Streptococcus lactis), homoacetic (Clostridium formicoaceticum), and acetate-utilizing methanogenic (Methanococcus mazei) bacteria was used to convert lactose and whey permeate to methane at mesophilic temperatures (35-37/sup 0/C) and a pH around 7.0. Lactose was first converted to lactic acid by S. lactis, then to acetic acid by C. formicoaceticum, and finally to methane and CO/sub 2/ by M. mazei. About 5.3 mol methane were obtained from each mole of lactose consumed, and the conversion of acetate to methane was the rate-limiting step for this mixed-culture fermentation.
- Research Organization:
- Purdue Univ., West Lafayette, IN (USA)
- OSTI ID:
- 6986655
- Journal Information:
- Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 32:1; ISSN BIBIA
- Country of Publication:
- United States
- Language:
- English
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ALKANES
BACTERIA
BIOCONVERSION
BIOREACTORS
BIOSYNTHESIS
CARBOHYDRATES
CLOSTRIDIUM
DATA
DISACCHARIDES
EXPERIMENTAL DATA
FERMENTATION
HYDROCARBONS
INDUSTRIAL WASTES
INFORMATION
LACTOSE
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
NUMERICAL DATA
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
RESOURCE POTENTIAL
SACCHARIDES
STREPTOCOCCUS
SYNTHESIS
WASTES
WHEY