Purification, characterization, and synergistic action of endoglucanases from Fusarium lini

Journal Article · Tue Jul 01 04:00:00 EDT 1986 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260280722· OSTI ID:6986386

Rao, M; Deshpande, V; Mishra, C

Five endoglucanses (1,4 - ..beta.. - d - glucan - glucanohydrolase, EC 3.2.1.4) were isolated from Fusarium lini. Endo 1 and 2 were purified by a preparative gel electrophoresis and Endo 3, 4, and 5 were purified in a single-step procedure involving preparative flat-bed isoelectric focusing. All the endoglucanases were homogenous on disk gel electrophoresis and analytical isoelectric focusing in polyacrylamide gel. The pl values were between 6 and 6.6 for Endo 3, 4, and 5; for Endo 1, the pl value was 8. The molecular weights of the enzymes were between 4 x 10/sup 4/ and 6.5 x 10/sup 4/. The Km values for endoglucanases using carboxymethyl cellulose (CM-cellulose) as the substrate were 2-12 mg/ml. The specificity of the enzymes was restricted to ..beta..-1,4-linkages. All the enzymes showed activity towards D-Xylan. The endoglucanases had high viscosity reducing activity with CM-cellulose. Striking synergism was observed for the hydrolysis of CM-cellulose by endoglucanases. Endo 2, 4 and 5 attacked cellopentaose and cellotetraose more readily than cellotriose. Endo 2 and 5 hydrolyzed cellotriose, cellotetraose, and cellopentaose, yielding a mixture of cellobiose with a trace amount of glucose; endo 4 produced only cellobiose. 23 references.

• Research Organization: National Chemical Lab., Poona, India
• OSTI ID: 6986386
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 28:7; ISSN BIBIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
CARBOHYDRATES
CELLOBIOSE
CELLULOLYTIC ACTIVITY
CELLULOSE
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DISACCHARIDES
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
FRACTIONATION
FUNGI
FUSARIUM
GEL PERMEATION CHROMATOGRAPHY
GLYCOSYL HYDROLASES
HYDROLASES
KINETICS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PARASITES
PLANTS
POLYSACCHARIDES
PURIFICATION
QUANTITATIVE CHEMICAL ANALYSIS
REACTION KINETICS
SACCHARIDES
SEPARATION PROCESSES
SUBSTRATES