Effect of ATPase inhibitors on cell potential and K/sup +/ influx in corn roots

Journal Article · Tue Jan 01 04:00:00 EST 1980 · Plant Physiol.; (United States)

OSTI ID:6984615

Cheeseman, J M; LaFayette, P R; Gronewald, J W; Hanson, J B

Experiments were performed to determine the effect of plasmalemma ATPase inhibitors on cell potentials (psi) and K/sup +/ (/sup 86/Rb) influx of corn root tissue over a wide range of K/sup +/ activity. N,N'Dicyclohexylcarbodiimide (DCCD), oligomycin, and diethylstilbestrol (DES) pretreatment greatly reduced active K/sup +/ influx and depolarized psi at low, but not at high, K/sup +/ activity (K/sup 0/). More comprehensive studies with DCCD and anoxia showed nearly complete inhibition of the active component of K/sup +/ influx over a wide range of K/sup 0/, with no effect on the apparent permeability constant. DCCD had no effect on the electrogenic component of the cell potential (psi/sub p/) above 0.2 millimolar K/sup 0/. Net proton efflux was rapidly reduced 80 to 90% by DCCD. Since tissue ATP content and respiration were only slightly affected by the DCCD-pretreatment, the inhibitions of active K/sup +/ influx and psi/sub p/ at low K/sup 0/ can be attributed to inhibition of the plasmalemma ATPase.

• Research Organization: Univ. of Illinois, Urbana
• OSTI ID: 6984615
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 65; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ACID ANHYDRASES
ALKALI METAL ISOTOPES
ALKALI METALS
ATP
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
CEREALS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
ELEMENTS
ENZYME INHIBITORS
ENZYMES
GRAMINEAE
GRASS
HYDROLASES
INHIBITION
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAIZE
METALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHOHYDROLASES
PLANTS
POTASSIUM
RADIOISOTOPES
ROOTS
RUBIDIUM 86
RUBIDIUM ISOTOPES
SOILS
TRACER TECHNIQUES