p-aminohippurate transport in the airways: Role of Na sup + and HCO sub 3 -

Journal Article · Fri Dec 01 04:00:00 EST 1989 · American Journal of Physiology; (USA)

OSTI ID:6983968

Cloutier, M M ^[1]

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1. Univ. of Connecticut Health Center, Farmington (USA)

The role of Na{sup +} and HCO{sub 3}- in the transport of p-aminohippurate (PAH) across the canine tracheal epithelium was investigated using Ussing chamber techniques and radiolabeled PAH. Under control conditions, net PAH absorption or a tendency toward net PAH absorption was observed. Neither amiloride (10(-4) M), furosemide (10(-3) M), ouabain (2 x 10(-4) M), nor Na+ substitution of the Ringer solution with choline had any effect on unidirectional PAH fluxes. When the Ringer solution was replaced with a HCO{sub 3}(-)-free solution, net PAH absorption was consistently observed. In HCO{sub 3}(-)-free experiments, unidirectional PAH absorptive fluxes were inhibited by mucosal addition of either of the stilbene derivatives, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS, 10(-4) M) or 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS, 10(-4) M). DIDS was more effective than SITS and was also effective in inhibiting PAH absorption in tissues bathed in Ringer solution. Submucosal DIDS or SITS had no effect on PAH fluxes either in HCO{sub 3}(-)-free or Ringer experiments. We conclude that PAH transport in canine tracheal epithelium occurs by a HCO{sub 3}(-)-PAH exchange process located on the luminal membrane. PAH transport is not Na{sup +} dependent but is inhibited by both DIDS and SITS.

• OSTI ID: 6983968
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 257; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID CARBONATES
ALKALI METAL COMPOUNDS
AMINO ACIDS
AMP
ANIMAL TISSUES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CELL MEMBRANES
DOGS
DRUGS
ELECTRIC CONDUCTIVITY
ELECTRICAL PROPERTIES
EPITHELIUM
GLYCOSIDES
HIPPURIC ACID
HYDROGEN COMPOUNDS
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
KINETICS
MAMMALS
MEMBRANE TRANSPORT
MEMBRANES
MUSCLES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OUABAIN
PHYSICAL PROPERTIES
REACTION KINETICS
RESPIRATORY SYSTEM
SODIUM COMPOUNDS
STROPHANTHINS
TISSUES
TRACER TECHNIQUES
TRACHEA
TRITIUM COMPOUNDS
VERTEBRATES