Gender-dependent problems in toxicity tests with Ceriodaphnia dubia
- Oak Ridge National Laboratory, TN (USA)
The 7-d, three-brood Ceriodaphnia chronic toxicity test developed by Mount and Norberg is used to assess biological quality of water and wastewaters. The test is becoming widely accepted because it involves simple procedures, uses easily measurable and biologically meaningful endpoints (survival and fecundity), and uses a microcrustacean known to be relatively sensitive to various toxicants. The test uses individual animals as true replicates, and is relatively powerful statistically. Because male and female Ceriodaphnia neonates cannot be readily distinguished from one another, a 7-d C. dubia test started with neonates produced by females from stressed cultures may include an initially indeterminant number of males. The presence of males (which can be ascertained with certainty only when the test is nearly complete) can affect interpretation of toxicity test results. In this paper the results of toxicity tests of water samples from streams near the Oak Ridge National Laboratory are used to show the extent to which the presence of male C. dubia can confound the interpretation of a 7-d Ceriodaphnia test's fecundity endpoint. The authors also show the results of short-term experiments suggesting that male C. dubia are more sensitive than females to cadmium and phenol, and discuss the statistical and practical implications of the problems caused by the presence of males in chronic toxicity tests with this species.
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 6983108
- Journal Information:
- Bulletin of Environmental Contamination and Toxicology; (USA), Journal Name: Bulletin of Environmental Contamination and Toxicology; (USA) Vol. 43:2; ISSN 0007-4861; ISSN BECTA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
AROMATICS
ARTHROPODS
BRANCHIOPODS
CADMIUM COMPOUNDS
CARCINOGEN SCREENING
CRUSTACEANS
DAPHNIA
ENVIRONMENTAL QUALITY
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INVERTEBRATES
LIQUID WASTES
MUTAGEN SCREENING
NATIONAL ORGANIZATIONS
ORGANIC COMPOUNDS
ORNL
OXYGEN COMPOUNDS
PHENOL
PHENOLS
POPULATION DYNAMICS
SCREENING
SEX DEPENDENCE
TOXICITY
US AEC
US DOE
US ERDA
US ORGANIZATIONS
WASTE WATER
WASTES
WATER
WATER QUALITY