Gonococcal attachment to eukaryotic cells
The attachment of Neisseria gonorrhoeae to eukaryotic cells grown in tissue culture was analyzed by use of light and electron microscopy and by labeling of the bacteria with (/sup 3/H)- and (/sup 14/C)adenine. Isogenic piliated and nonpiliated N. gonorrhoeae from opaque and transparent colonies were studied. The results of light microscopy studies showed that the gonococci attached to cells of human origin, including Flow 2000, HeLa 229, and HEp 2. Studies using radiolabeled gonococci gave comparable results. Piliated N. gonorrhoeae usually attached in larger numbers than nonpiliated organisms, and those from opaque colonies attached more often than isogenic variants from transparent colonies. Day-to-day variation in rate of attachment was observed. Scanning electron microscopy studies showed the gonococcal attachment to be specific for microvilli of the host cells. It is concluded that more N. gonorrhoeae from opaque colonies, as compared with isogenic variants from transparent colonies, attach to eukaryotic cells grown in tissue culture.
- Research Organization:
- Univ. of California, San Francisco
- OSTI ID:
- 6981374
- Journal Information:
- Sex. Transm. Dis.; (United States), Vol. 10:4
- Country of Publication:
- United States
- Language:
- English
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BACTERIA
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TRACER TECHNIQUES
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FIBROBLASTS
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ANIMAL CELLS
BACTERIAL DISEASES
CONNECTIVE TISSUE CELLS
DISEASES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
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UROGENITAL SYSTEM DISEASES
550701* - Microbiology- Tracer Techniques