Deuterium-labeling method for the assignment of histidine nuclear magentic resonance peaks of proteins

Journal Article · Wed May 10 04:00:00 EDT 1978 · J. Am. Chem. Soc.; (United States)

DOI:https://doi.org/10.1021/ja00478a054· OSTI ID:6977006

Arata, Y ^[1]; Shimizu, A; Matsuo, H

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1. Tokyo Univ.

A tritium labelling method involving differential tritium exchange at the C-2 H position of histidines and /sup 1/H NMR of differentially deuterated proteins can be a general method for the assignment of the histidine NMR peaks. In the present report this method is modified by replacing the tritium with deuterium, which eliminates ambiguities arising from the tritium isotope effect. In the deuterium labelling method, differentially deuterated proteins are cleaved by trypsin into smaller peptides each containing a single histidine residue, which are separated by chromatography. The method was applied to the Bence Hones dimer Ak which contains two histidine residues in the constant domain of each of the light chains. The decay of the NMR peaks with time allowed the assignment of one peak to His/sup 189/ and the other to His/sup 198/. (JSR)

• OSTI ID: 6977006
• Journal Information: J. Am. Chem. Soc.; (United States), Journal Name: J. Am. Chem. Soc.; (United States) Vol. 100:10; ISSN JACSA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400303* -- Organic Chemistry-- Isotope Exchange & Isotope Separation-- (-1987)
AMINO ACIDS
AZOLES
CARBOXYLIC ACIDS
DEUTERIUM
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
HYDROGEN ISOTOPES
IMIDAZOLES
ISOTOPES
ISOTOPIC EXCHANGE
LABELLING
LIGHT NUCLEI
MAGNETIC RESONANCE
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
RESONANCE
STABLE ISOTOPES