Archaeoglobus fulgidus isolated from hot north sea oil field waters
- Univ. of Bergen, Bergen (Norway)
A hyperthermophilic sulfate reducer, strain 7324, was isolated from hot (75[degrees]C) oil field waters from an oil production platform in the Norwegian sector of the North Sea. It was enriched on a complex medium and isolated on lactate with sulfate. The cells were nonmotile, irregular coccoid to disc shaped, and 0.3 to 1.0 [mu]m wide. The temperature for growth was between 60 and 85[degrees]C with an optimum of 76[degrees]C. Lactate, pyruvate, and valerate plus H[sub 2] were utilized as carbon and energy sources with sulfate as electron acceptor. Lactate was completely oxidized to CO[sub 2]. The cells contained an active carbon monoxide dehydrogenase but no 2-oxoglutarate dehydrogenase pathway. The cells produced small amounts of methane simultaneously with sulfate reduction. F[sub 420] was detected in the cells which showed a blue-green fluorescence at 420 nm. On the basis of morphological, physiological, and serological features, the isolate was classified as an Archaeoglobus sp. Strain 7324 showed 100% DNA-DNA homology with A. fulgidus Z, indicating that it belongs to the species A. fulgidus. Archaeoglobus sp. has been selectively enriched and immunomagnetically captured from oil fields waters from three different platforms in the North Sea. Our results show that strain 7324 may grow in oil reservoirs at 70 to 85[degrees]C and contribute to hydrogen sulfide formation in this environment. 37 refs., 2 figs.
- OSTI ID:
- 6976039
- Journal Information:
- Applied and Environmental Microbiology; (United States), Vol. 60:4; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
GROWTH
NORTH SEA
OIL FIELDS
THERMOPHILIC CONDITIONS
SULFATES
REDUCTION
ENERGY SOURCES
LACTATES
ATLANTIC OCEAN
CARBOXYLIC ACID SALTS
CHEMICAL REACTIONS
GEOLOGIC DEPOSITS
MICROORGANISMS
MINERAL RESOURCES
OXYGEN COMPOUNDS
PETROLEUM DEPOSITS
RESOURCES
SEAS
SULFUR COMPOUNDS
SURFACE WATERS
550200* - Biochemistry
560300 - Chemicals Metabolism & Toxicology