Isolation and identification of mutagenic polycyclic aromatic hydrocarbons from a coal gasifier condensate
An iterative scheme of short-term bacterial mutagenesis assays and multidimensional liquid chromatographic methods was used to isolate mutagens from a neutral fraction of a complex carcinogenic tar formed as a by-product of coal gasification. Mutagens in the complementary reversed- and normal-phase liquid chromatographic fractions were identified by capillary column gas chromatography and gas chromatography-mass spectrometry. The principal mutagens included a variety of 4- to 6-ring unsubstituted polycyclic aromatic hydrocarbons, their alkylated homologs, and related methylene-bridged compounds. Most of the unsubstituted mutagens were known carcinogens that were qualitatively similar to those detected by carcinogenicity assays of fractions from other types of coal tars and cigarette smoke condensate. The chromatographic approach is generally applicable to complex mixtures and is especially well suited for fractionating mixtures of aromatic compounds and their alkyl homologs.
- Research Organization:
- Argonne National Lab., IL
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 6975615
- Journal Information:
- Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 41:2; ISSN ENVRA
- Country of Publication:
- United States
- Language:
- English
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010600* -- Coal
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560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
CARCINOGENS
CHROMATOGRAPHY
COAL GASIFICATION
COAL TAR
GAS CHROMATOGRAPHY
GASIFICATION
HYDROCARBONS
MASS SPECTROSCOPY
MICROORGANISMS
MUTAGENESIS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
SEPARATION PROCESSES
SPECTROSCOPY
TAR
THERMOCHEMICAL PROCESSES