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Title: The human granzyme A (HFSP, CTLA3) gene maps to 5q11-q12 and defines a new locus of the serine protease superfamily

Abstract

Human granzyme A (HFSP, Hanukah factor serine protease; CTLA3, cytotoxic T-lymphocyte-associated serine esterase-3), a homodimeric, trypsin-like serine protease of 60 kDa found in granules of cytolytic T cells and natural killer cells, is implicated in lymphocyte-mediated target cell lysis. It contributes to DNA fragmentation in perforin (PRF1)-lysed target cells through an unknown mechanism. The authors have isolated a cosmid clone for the functional gene of human granzyme A and established its complete exon-intron map of 10 kb. Using an 11-kb subfragment of the cloned genomic DNA as a probe, they have identified the chromosomal position of human granzyme A on 5q11-q12. Thus, the human granzyme A gene falls into a region of homology between human chromosome 5 and mouse chromosome 13, band D, where the mouse granzyme A gene has been located previously. The granzyme A gene is not linked to known members of the large superfamily of serine proteases. 20 refs., 2 figs.

Authors:
;  [1]; ; ;  [2]
  1. (Institut fuer angewandte Tumorvirologie, Heidelberg (Germany))
  2. (Max-Planck-Institut fuer Psychiatrie, Planegg-Martinsried (Germany))
Publication Date:
OSTI Identifier:
6975050
Resource Type:
Journal Article
Resource Relation:
Journal Name: Genomics; (United States); Journal Volume: 18:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HUMAN CHROMOSOME 5; GENETIC MAPPING; SERINE PROTEINASES; GENES; CELL KILLING; DNA SEQUENCING; DNA-CLONING; CHROMOSOMES; CLONING; DNA HYBRIDIZATION; ENZYMES; HUMAN CHROMOSOMES; HYBRIDIZATION; HYDROLASES; MAPPING; ORGANIC COMPOUNDS; PEPTIDE HYDROLASES; PROTEINS; STRUCTURAL CHEMICAL ANALYSIS; 550400* - Genetics

Citation Formats

Fink, T.M., Lichter, P., Wekerle, H., Zimmer, M., and Jenne, D.E. The human granzyme A (HFSP, CTLA3) gene maps to 5q11-q12 and defines a new locus of the serine protease superfamily. United States: N. p., 1993. Web. doi:10.1006/geno.1993.1483.
Fink, T.M., Lichter, P., Wekerle, H., Zimmer, M., & Jenne, D.E. The human granzyme A (HFSP, CTLA3) gene maps to 5q11-q12 and defines a new locus of the serine protease superfamily. United States. doi:10.1006/geno.1993.1483.
Fink, T.M., Lichter, P., Wekerle, H., Zimmer, M., and Jenne, D.E. 1993. "The human granzyme A (HFSP, CTLA3) gene maps to 5q11-q12 and defines a new locus of the serine protease superfamily". United States. doi:10.1006/geno.1993.1483.
@article{osti_6975050,
title = {The human granzyme A (HFSP, CTLA3) gene maps to 5q11-q12 and defines a new locus of the serine protease superfamily},
author = {Fink, T.M. and Lichter, P. and Wekerle, H. and Zimmer, M. and Jenne, D.E.},
abstractNote = {Human granzyme A (HFSP, Hanukah factor serine protease; CTLA3, cytotoxic T-lymphocyte-associated serine esterase-3), a homodimeric, trypsin-like serine protease of 60 kDa found in granules of cytolytic T cells and natural killer cells, is implicated in lymphocyte-mediated target cell lysis. It contributes to DNA fragmentation in perforin (PRF1)-lysed target cells through an unknown mechanism. The authors have isolated a cosmid clone for the functional gene of human granzyme A and established its complete exon-intron map of 10 kb. Using an 11-kb subfragment of the cloned genomic DNA as a probe, they have identified the chromosomal position of human granzyme A on 5q11-q12. Thus, the human granzyme A gene falls into a region of homology between human chromosome 5 and mouse chromosome 13, band D, where the mouse granzyme A gene has been located previously. The granzyme A gene is not linked to known members of the large superfamily of serine proteases. 20 refs., 2 figs.},
doi = {10.1006/geno.1993.1483},
journal = {Genomics; (United States)},
number = ,
volume = 18:2,
place = {United States},
year = 1993,
month =
}
  • This submission in the Gene Antigen Register section of Immunogenetics journal describes research in which fluorescence in situ hybridization (FISH) was performed to map and determine independently the precise location of the human tryptase-2 (TRYP2) and human granzyme A (HFSP) genes. The results indicate that both genes map to chromosome 5q11-q12 and define a new locus for cytotoxic lymphocyte granule tryptases. 14 refs, 2 figs.
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  • The chymotrypsin-like family of serine protease genes includes several members that are expressed exclusively in subsets of hematopoietic cells. For example, human neutrophil elastase and cathepsin G are expressed only in myelomonocytic precursors, and cytotoxic-T-cell serine proteases are found only in cytotoxic lymphocytes. The authors have used a cathepsin G cDNA probe to clone two cathepsin G-like genes (designated CGL-1 and CGL-2) from a human genomic library. They have determined that CGL-1 is identical to a previously identified gene (known as CCPI, CTLA I, or cytotoxic serine protease B) that is expressed only in activated cytotoxic T lymphocytes. They showmore » here that cathepsin G, CGL-1, and CGL-2 are linked on an {approx}50-kilobase locus found on human chromosome 14 at band q11.2. This gene cluster maps to the same chromosomal band as the {alpha} and {delta} T-cell receptor genes; this region is involved in most chromosomal translocations and inversions that are specifically associated with T-cell malignancies.« less
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