The human granzyme A (HFSP, CTLA3) gene maps to 5q11-q12 and defines a new locus of the serine protease superfamily

Journal Article · Mon Nov 01 04:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1483· OSTI ID:6975050

Fink, T M; Lichter, P ^[1]; Wekerle, H; Zimmer, M; Jenne, D E ^[2]

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1. Institut fuer angewandte Tumorvirologie, Heidelberg (Germany)
2. Max-Planck-Institut fuer Psychiatrie, Planegg-Martinsried (Germany)

Human granzyme A (HFSP, Hanukah factor serine protease; CTLA3, cytotoxic T-lymphocyte-associated serine esterase-3), a homodimeric, trypsin-like serine protease of 60 kDa found in granules of cytolytic T cells and natural killer cells, is implicated in lymphocyte-mediated target cell lysis. It contributes to DNA fragmentation in perforin (PRF1)-lysed target cells through an unknown mechanism. The authors have isolated a cosmid clone for the functional gene of human granzyme A and established its complete exon-intron map of 10 kb. Using an 11-kb subfragment of the cloned genomic DNA as a probe, they have identified the chromosomal position of human granzyme A on 5q11-q12. Thus, the human granzyme A gene falls into a region of homology between human chromosome 5 and mouse chromosome 13, band D, where the mouse granzyme A gene has been located previously. The granzyme A gene is not linked to known members of the large superfamily of serine proteases. 20 refs., 2 figs.

• OSTI ID: 6975050
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 18:2; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CELL KILLING
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ENZYMES
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 5
HUMAN CHROMOSOMES
HYBRIDIZATION
HYDROLASES
MAPPING
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PROTEINS
SERINE PROTEINASES
STRUCTURAL CHEMICAL ANALYSIS