Cloning of cDNAs that encode human mast cell carboxypeptidase A, and comparison of the protein with mouse mast cell carboxypeptidase A and rat pancreatic carboxypeptidases

Journal Article · Fri Dec 01 04:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.23.9480· OSTI ID:6970212

Reynolds, D S; Gurley, D S; Stevens, R L; Austen, K F; Serafin, W E ^[1]; Sugarbaker, D J ^[2]

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1. Harvard Medical School, Cambridge, MA (USA) Brigham and Women's Hospital, Boston, MA (USA)
2. Harvard Medical School, Cambridge, MA (USA)

Human skin and lung mast cells and rodent peritoneal cells contain a carboxypeptidase in their secretory granules. The authors have screened human lung cDNA libraries with a mouse mast cell carboxypeptidase A (MC-CPA) cDNA probe to isolate a near-full-length cDNA that encodes human MC-CPA. The 5{prime} end of the human MC-CPA transcript was defined by direct mRNA sequencing and by isolation and partial sequencing of the human MC-CPA gene. Human MC-CPA is predicted to be translated as a 417 amino acid preproenzyme which includes a 15 amino acid signal peptide and a 94-amino acid activation peptide. The mature human MC-CPA enzyme has a predicted size of 36.1 kDa, a net positive charge of 16 at neutral pH, and 86% amino acid sequence identity with mouse MC-CPA. DNA blot analyses showed that human MC-CPA mRNA is transcribed from a single locus in the human genome. Comparison of the human MC-CPA with mouse MC-CPA and with three rat pancreatic carboxypeptidases shows that these enzymes are encoded by distinct but homologous genes.

• OSTI ID: 6970212
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:23; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYPEPTIDASES
CLONING
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ENDOCRINE GLANDS
ENZYMES
GENES
GLANDS
HYBRIDIZATION
HYDROLASES
IMMUNOLOGY
ISOTOPES
LIGHT NUCLEI
LUNGS
MAMMALS
MAN
MAST CELLS
MESSENGER-RNA
MICE
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDE HYDROLASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
RATS
RECOMBINANT DNA
RESPIRATORY SYSTEM
RNA
RODENTS
SKIN
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES