Stimulation of DNA synthesis in human epidermis by UVB radiation and its inhibition by difluoromethylornithine

Journal Article · Sun Apr 01 04:00:00 EDT 1990 · Plastic and Reconstructive Surgery; (USA)

DOI:https://doi.org/10.1097/00006534-199004000-00015· OSTI ID:6969684

Eshbaugh, Jr, W G; Forley, B G; Ritter, E F; Serafin, D; Klitzman, B ^[1]

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1. Duke Univ. Medical Center, Durham, NC (USA)

The purpose of this study was to determine whether the rate of DNA synthesis in human skin could be increased by UVB radiation and to determine the potential for reversing the stimulatory effects of UVB radiation by alpha-difluoromethylornithine (DFMO). Split-thickness facial skin was grafted onto athymic CD-1 Nu/Nu mice on the anterolateral dorsal surface. Following graft healing for 6 weeks, grafts were treated with 0%, 2%, or 5% DFMO (a potent inhibitor of polyamine biosynthesis) and subsequently irradiated with 0.15 J/cm2 of UVB light. Two days after UVB exposure, ({sup 3}H)thymidine was injected and the grafts were dissected and counted. Ultraviolet radiation significantly increased thymidine incorporation, indicating increased DNA synthesis. The stimulatory effects of UV radiation were significantly reduced by topical application of 5% DFMO. Thus administration of DFMO most likely decreased the polyamine level and decreased the rate of DNA synthesis, which may have caused a decreased rate of epidermal proliferation. Thus the topical application of DFMO may prove beneficial for UVB exposure and other hyperproliferative states where a decrease in the rate of cell turnover might be desirable.

• OSTI ID: 6969684
• Journal Information: Plastic and Reconstructive Surgery; (USA), Journal Name: Plastic and Reconstructive Surgery; (USA) Vol. 85:4; ISSN PRSUA; ISSN 0032-1052
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBOXYLIC ACIDS
CELL PROLIFERATION
DNA REPLICATION
ELECTROMAGNETIC RADIATION
EPIDERMIS
EPITHELIUM
GRAFTS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
MAN
MICE
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
ORNITHINE
PRIMATES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RIBOSIDES
RODENTS
SKIN
THYMIDINE
TISSUES
TRACER TECHNIQUES
TRANSPLANTS
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
VERTEBRATES