In vivo sup 23 Na and sup 31 P NMR measurement of a tonoplast Na sup + /H sup + exchange process and its characteristics in two barley cultivars

Journal Article · Fri Dec 01 00:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.24.9856· OSTI ID:6969404

Fan, T W.M.; Norlyn, J; Epstein, E ^[1]; Higashi, R M ^[2]

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1. Univ. of California, Davis (USA)
2. Univ. of California, Bodega Bay (USA)

A Na{sup +} uptake-associated vacuolar alkalinization was observed in roots of two barley cultivars (Arivat and the more salt-tolerant California Mariout) by using {sup 23}Na and {sup 31}P in vivo NMR spectroscopy. A NaCl uptake-associated broadening was also noted for both vacuolar P{sub i} and intracellular Na NMR peaks, consistent with Na{sup +} uptake into the same compartment as the vacuolar P{sub i}. A close coupling of Na{sup +} with H{sup +} transport (presumably the Na{sup +}/H{sup +} antiport) in vivo was evidence by qualitative and quantitative correlations between Na{sup +} accumulation and vacuolar alkalinization for both cultivars. Prolongation of the low NaCl pretreatment (30 mM) increased the activity of the putative antiport in Arivat but reduced it in California Mariout. This putative antiport also showed a dependence on NaCl concentration for California Mariout but not for Arivat. No cytoplasmic acidification accompanied the antiporter activity for either cultivar. The response of adenosine phosphates indicated that ATP utilization exceeded the capacity for ATP synthesis in Arivat, but the two processes seemed balanced in California Mariout. These comparisons provide clues to the role of the tonoplast Na{sup +}/H{sup +} antiport and compensatory cytoplasmic adjustments including pH, osymolytes, and energy phosphates in governing the different salt tolerance of the two cultivars.

• OSTI ID: 6969404
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:24; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
IONS
MEMBRANE TRANSPORT
MEMBRANE PROTEINS
NUCLEAR MAGNETIC RESONANCE
ATP
BARLEY
PH VALUE
PHOSPHORUS 31
PROTONS
SEEDS
SODIUM 23
SODIUM CHLORIDES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
BARYONS
CEREALS
CHARGED PARTICLES
CHLORIDES
CHLORINE COMPOUNDS
ELEMENTARY PARTICLES
FERMIONS
GRASS
HADRONS
HALIDES
HALOGEN COMPOUNDS
ISOTOPES
LIGHT NUCLEI
LILIOPSIDA
MAGNETIC RESONANCE
MAGNOLIOPHYTA
NUCLEI
NUCLEONS
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PLANTS
PROTEINS
RESONANCE
SODIUM COMPOUNDS
SODIUM ISOTOPES
STABLE ISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics