Analysis of the synthetic pyrethroids, permethrin and 1(R)-phenothrin, in grain using a monoclonal antibody-based test

Journal Article · Wed Jul 01 00:00:00 EDT 1992 · Journal of Agricultural and Food Chemistry; (United States)

DOI:https://doi.org/10.1021/jf00019a041· OSTI ID:6969124

Skerritt, J H; Hill, A S ^[1]; McAdam, D P ^[2]; Stanker, L H ^[3]

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1. CSIRO Division of Plant Industry, North Ryde (Australia)
2. CSIRO Division of Plant Industry, Canberra (Australia)
3. University of California, Livermore (United States)

A monoclonal antibody generated to the synthetic pyrethroid-related hapten, (3-phenoxybenzyl)-2,2-dimethylcyclopropane-1, 3-dicarboxylate-protein conjugate, was used to develop assays for determinations of permethrin and 1(R)-phenothrin in wheat grain and flour milling fractions. The earlier 3-h assay was simplified using two approaches. The antibody was directly conjugated to the enzyme horseradish peroxidase (HRP), which removes a separate incubation and washing step from the assay. Also, an assay has been developed using microwell-bound monoclonal antibody and a HRP-labeled 3-phenoxybenzoic acid derivative. These assay formats have advantages in increased sensitivity and, in the case of the latter assay, accuracy with grain and flour samples. The most sensitive assay format could detect 1.5 ng/mL permethrin; 50% inhibition of antibody binding occurred at 10 ng/mL. These values corresponded to 75 and 500 ppb, respectively, in the original wheat sample. Methanol was the most effective pyrethroid extractant. Use of a simple cleanup procedure for ground grain extracts improved ELISA accuracy but could by omitted for screening purposes.

• OSTI ID: 6969124
• Journal Information: Journal of Agricultural and Food Chemistry; (United States), Journal Name: Journal of Agricultural and Food Chemistry; (United States) Vol. 40:7; ISSN 0021-8561; ISSN JAFCAU
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AGRICULTURE
ANTIBODIES
BIOASSAY
CEREALS
DISINFESTATION
ENZYME IMMUNOASSAY
ENZYMES
EXTRACTION
GRAIN DISINFESTATION
GRAMINEAE
IMMUNOASSAY
INSECTICIDES
LILIOPSIDA
MAGNOLIOPHYTA
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
PESTICIDES
PLANTS
PROTEINS
SEPARATION PROCESSES
SOLVENT EXTRACTION