[Molecular, genetic and physiological analysis of photoinhibition and photosynthetic]
A major goal of this project is to use a combined molecular genetic, biochemical and physiological approach to understand the relationship between photosynthetic performance and the structure of the multifunctional D1 reaction center protein of Photosystem II encoded by the chloroplast psbA gene. Relative to other chloroplast proteins, turover of D1 is rapid and highly light dependent and de novo synthesis of D1 is required for a plant's recovery from short term exposure to irradiances which induce photoinhibitory damage. These observations have led to models for a damage/repair cycle of PSII involving the targeted degradation and replacement of photodamaged D1. To investigate the effects of perturbing the D1 cycle on photosynthesis and autotrophic growth under high and low irradiance, we have examined the consequences of site-specific mutations of the psbA and 16S rRNA genes affecting synthesis, maturation and function/stability of the D1 protein introduced into the chloroplast genome of wildtype strain of the green alga Chlamydomonas reinhardtii using biolistic transformation.
- Research Organization:
- Duke Univ., Durham, NC (United States)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG05-89ER14005
- OSTI ID:
- 6969011
- Report Number(s):
- DOE/ER/14005-T2; ON: DE93004365
- Country of Publication:
- United States
- Language:
- English
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THYLAKOID MEMBRANE PROTEINS
GENE MUTATIONS
PHOSPHORYLATION
BIOLOGICAL HALF-LIFE
CARBON 13
DNA SEQUENCING
MUTANTS
OXYGEN 18
PHOTOSYNTHETIC REACTION CENTERS
PROGRESS REPORT
RIBOSOMAL RNA
RNA PROCESSING
SULFUR 35
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON ISOTOPES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
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EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MEMBRANE PROTEINS
MUTATIONS
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXYGEN ISOTOPES
PROTEINS
RADIOISOTOPES
RNA
STABLE ISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
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