Recombinant tumor necrosis factor. alpha. and interleukin 1. alpha. increase expression of c-abl protooncogene mRNA in cultured human marrow stromal cells

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.17.6788· OSTI ID:6968883

Andrews, III, D F; Nemunaitis, J J; Singer, J W ^[1]

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1. Univ. of Washington, Seattle, WA (USA)

Analysis of protooncogene RNA expression in marrow stromal cells from long-term marrow culture demonstrated high levels of c-abl 5-, 6-, and 7-kilobase (kb) RNA transcripts. In experiments on three independently derived simian virus 40-transformed marrow stromal cell lines, the expression of these c-abl transcripts was further increased in response to recombinant tumor necrosis factor {alpha} (1,000 units/ml) and interleukin 1{alpha} (10 units/ml). Although lymphocyte-conditioned medium predominantly up-regulated the 5-kb transcript, interleukin 1{alpha} primarily affected the 6-kb transcript. The up-regulation of the 5-kb c-abl message correlated with up-regulation of the granulocyte/macrophage colony-stimulating factor transcript and down-regulation of procollagen I transcripts in transformed cells. These data suggest that c-abl plays roles in the regulation of extracellular matrix expression and in the regulation of hematopoietic growth factors by stromal cells.

• OSTI ID: 6968883
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:17; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BONE MARROW CELLS
CELL DIFFERENTIATION
LYMPHOKINES
BIOLOGICAL EFFECTS
ONCOGENES
CHEMICAL ACTIVATION
COLLAGEN
DNA HYBRIDIZATION
GENE REGULATION
HETEROCHROMOSOMES
MACROPHAGES
MESSENGER-RNA
NECROSIS
TRANSCRIPTION
ANIMAL CELLS
CHROMOSOMES
CONNECTIVE TISSUE CELLS
GENES
GROWTH FACTORS
HYBRIDIZATION
MITOGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PHAGOCYTES
PROTEINS
RNA
SCLEROPROTEINS
SOMATIC CELLS
550201* - Biochemistry- Tracer Techniques