Detection of murine cytomegalovirus DNA in circulating leukocytes harvested during acute infection of mice

Journal Article · Thu Jun 01 00:00:00 EDT 1989 · Journal of Virology; (USA)

OSTI ID:6968882

Bale, Jr, J F; O'Neil, M E ^[1]

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1. Univ. of Iowa College of Medicine, Iowa City (USA)

The authors used virus assay and in situ hybridization with a cloned fragment of the murine cytomegalovirus (MCMV) genome to study MCMV infection of circulating leukocytes harvested from 3-week-old BALB/c, C57BL/6, and C3H mice infected with MCMV intraperitoneally. Infectious virus or MCMV DNA was detected in leukocytes on days 1 through 21 of infection in BALB/c mice and on days 3 through 7 in C57BL/6 mice. On days 5 and 7, MCMV DNA or infectious virus was detected in the leukocytes of 17 (94%) of 18 BALB/c mice and 10 (59%) of 17 C57BL/6 mice. In both strains infection peaked on days 5 and 7, when as many as 0.01 to 0.1% of the circulating leukocytes contained MCMV DNA. In C3H mice, however, infectious virus was rarely recovered from leukocyte fractions and MCMV DNA was detected in the circulating leukocytes of only one animal. Circulating leukocytes may have an important role in the dissemination of CMV infections in susceptible hosts.

• OSTI ID: 6968882
• Journal Information: Journal of Virology; (USA), Vol. 63:6; ISSN 0022-538X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LEUKOCYTES
MOLECULAR BIOLOGY
VIRAL DISEASES
DIAGNOSIS
VIRUSES
DNA HYBRIDIZATION
DNA
MAN
MICE
NUCLEOTIDES
SULFUR 35
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
HYBRIDIZATION
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PRIMATES
RADIOISOTOPES
RODENTS
SULFUR ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques