Benzo[a]pyrene (BaP) metabolites and 1-hydroxypyrene as urinary biomarkers for human environmental exposure to BaP

Thesis/Dissertation · Tue Jan 01 04:00:00 EST 1991

OSTI ID:6968546

Buckley, T J

Environmental exposures to benzo[a]pyrene (BaP) through inhalation and ingestion pathways and the corresponding elimination of BaP metabolites in the urine were evaluated through regression and mass balance models. Personal air sampling (24-h) yielded BaP inhalation dose estimates of 11.0 and 2.3 ng/d for winter and late summer, respectively, while duplicate plate sampling yielded an ingested dose estimate of 176 ng/d. The daily inhalation dose was exceeded by the ingested dose by median factors of 6 and 122 for the respective study phases. Using the reverse metabolism method of analysis, median urinary BaP metabolite elimination rates were 121 and 129 ng/d for Phase 2 and 3, respectively. BaP dose (inhalation and ingestion) and urinary BaP metabolite elimination variables were related using multiple linear regression. When the difference between week 1 and 2 in dose and metabolite elimination was considered, and when body weight was included, a statistically significant model was determined (r = 0.620, p = 0.015, n = 25) with most of the variation in urine elimination being explained by ingested dose. A separate controlled exposure study was conducted among five nonsmoking males to define the time-course of urinary 1-hydroxypyrene (1-OHPY) elimination associated with ingestion of foods containing polycyclic aromatic hydrocarbons (PAHs). This investigation showed that a 100-250 fold increase in the dietary BaP does paralleled to 4 to 12 fold increase in the elimination of 1-OHPY. A biexponential model was fitted to the 1-OHPY elimination rate data yielding an average half-life estimate of 4.4 h and an average time of maximal rate of elimination of 6.3 h after the ingested dose.

• Research Organization: Rutgers--the State Univ., New Brunswick, NJ (United States)
• OSTI ID: 6968546
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BENZOPYRENE
BIOLOGICAL MARKERS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CONDENSED AROMATICS
ENVIRONMENTAL EXPOSURE
ENVIRONMENTAL EXPOSURE PATHWAY
HYDROCARBONS
INGESTION
INHALATION
INTAKE
MATERIALS
MATHEMATICAL MODELS
MATHEMATICS
METABOLISM
ORGANIC COMPOUNDS
REGRESSION ANALYSIS
STATISTICS
URINE
WASTES