Independent prediction of naphthalene transport and biodegradation in soil with a mathematical model

Journal Article · Tue Oct 05 04:00:00 EDT 1999 · Biotechnology and Bioengineering

DOI:https://doi.org/10.1002/(SICI)1097-0290(19991005)65:1<65::AID-BIT8>3.0.CO;2-K· OSTI ID:696816

Ahn, I S; Ghiorse, W C; Lion, L W; Shuler, M L ^[1]

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1. Cornell Univ., Ithaca, NY (United States)

Experiments were performed to test the ability of a mathematical model to predict naphthalene transport and biodegradation. Pseudomonas putida G7, a model bacterial strain capable of degrading naphthalene, was added to a column packed with the soil that had been pre-equilibrated with naphthalene. Model prediction for transport and degradation were based on predetermined parameters that described naphthalene desorption kinetics and the utilization of naphthalene by the test bacterium. However, initial prediction for naphthalene biodegradation was high, and the formation of cell aggregates is advances as a plausible explanation. Access of substrate to cells in the interior of an aggregate would be restricted. When the numerical simulation was conducted with a factor to account for cell aggregation, it successfully described the experimental data. Thus, with a single adjustable parameter (an average effectiveness factor), the model predicted macroscopic responses of naphthalene in soil-columns where naphthalene was subject to transport and biodegradation.

• OSTI ID: 696816
• Journal Information: Biotechnology and Bioengineering, Journal Name: Biotechnology and Bioengineering Journal Issue: 1 Vol. 65; ISSN BIBIAU; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
BIODEGRADATION
ENVIRONMENTAL TRANSPORT
MATHEMATICAL MODELS
NAPHTHALENE
PSEUDOMONAS
REMEDIAL ACTION
SOILS