Big Blue Transgenic Mouse lacl mutation analysis

Journal Article · Thu Dec 31 23:00:00 EST 1992 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850220303· OSTI ID:6963820

Stiegler, G L; Stillwell, L C ^[1]

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1. Pacific Northwest Laboratory, Richland, WA (United States)

In this report the authors describe a rapid general method for mutant blue plaque molecular analysis. The mutant analysis discussed here resulted from radon inhalation exposure. The described method circumvents Stratagene's plasmid isolation and ensuing sequence analysis of the entire lac1 gene. The authors have adapted the polymerase chain reaction (PCR) method and single-strand conformation polymorphism (SSCP) analysis for localizing mutations within the lac1 coding region. Three overlapping PCR products of approximately 450 bp representing the entire lac1 coding region are used for SSCP analysis. Those PCR products with an altered SSCP electrophoretic migration focus the mutation to a smaller region of the lac1 gene that is analyzed by direct cycle sequencing. 5 refs., 1 fig.

• DOE Contract Number: AC06-76RL01830
• OSTI ID: 6963820
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 22:3; ISSN 0893-6692; ISSN EMMUEG
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOASSAY
DETECTION
DNA SEQUENCING
ELEMENTS
FLUIDS
GASES
GENE MUTATIONS
INHALATION
INTAKE
MAMMALS
MICE
MUTATIONS
NONMETALS
RADON
RARE GASES
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
TRANSGENIC ANIMALS
TRANSGENIC MICE
VERTEBRATES