Tritiated-nicotine and /sup 125/I-alpha-bungarotoxin-labeled nicotinic receptors in the interpeduncular nucleus of rats. I. Subnuclear distribution

Journal Article · Mon Sep 15 04:00:00 EDT 1986 · J. Comp. Neurol.; (United States)

DOI:https://doi.org/10.1002/cne.902510310· OSTI ID:6963799

Hamill, G S; Clarke, P B; Pert, A; Jacobowitz, D M

The distribution of nicotinic receptors within the interpeduncular nucleus (IPN) was determined in male rats following in vitro labeling with the cholinergic ligands /sup 3/H-nicotine and /sup 125/I-alpha-bungarotoxin (BTX). Autoradiographic images of two rostrocaudal levels of IPN were analyzed by computer-assisted densitometry and the optical density contributed by displaceable labeling was determined in the rostral, central, intermediate, and lateral subnuclei. /sup 3/H-nicotine labeling density within the four subnuclei differs significantly at both levels of IPN. The greatest density of labeling is localized in the rostral subnucleus, followed in order of diminishing density by the central, intermediate, and lateral subnuclei. Labeling within the rostral subnucleus is prominently localized within its central zone. In the central subnucleus, a dense concentration of binding sites is apparent in the middle region, adjacent to less dense vertically oriented columns; /sup 3/H-nicotine binding sites in the lateral subnuclei appear to be most concentrated medially, adjacent to the intermediate subnuclei. /sup 125/I-BTX labeling density within the four subnuclei also differs significantly at both levels of IPN. The greatest density of labeling is found in the rostral subnucleus, followed in order of decreasing density by the lateral, central, and intermediate subnuclei. The ovoid regions of the rostral subnucleus contain dense /sup 125/I-BTX labeling. In the lateral subnuclei, /sup 125/I-BTX binding appears to be predominantly along the lateral margins of the subnucleus. The present data indicate that the IPN contains two distinct populations of putative cholinergic nicotinic receptors identified, respectively, by /sup 3/H-nicotine and /sup 125/I-BTX labeling. Each population of labeled receptors is uniquely localized in patterns that suggest differences in density within and across subnuclei.

• Research Organization: National Institute of Mental Health, Bethesda, MD
• OSTI ID: 6963799
• Journal Information: J. Comp. Neurol.; (United States), Journal Name: J. Comp. Neurol.; (United States) Vol. 3; ISSN JCNEA
• Country of Publication: United States
• Language: English

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551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALOIDS
AMINES
ANIMAL CELLS
ANIMALS
ANTIGENS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
AZINES
AZOLES
BETA DECAY RADIOISOTOPES
BODY
BRAIN
CELL CONSTITUENTS
CELL NUCLEI
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
NERVE CELLS
NERVOUS SYSTEM
NICOTINE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARASYMPATHOLYTICS
PARASYMPATHOMIMETICS
PROTEINS
PYRIDINES
PYRROLES
PYRROLIDINES
RADIOISOTOPES
RADIORECEPTOR ASSAY
RATS
RECEPTORS
RODENTS
SOMATIC CELLS
TISSUE DISTRIBUTION
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES