Affinity purifications of aldose reductase and xylitol dehydrogenase from the xylose-fermenting yeast Pachysolen tannophilus
Journal Article
·
· Appl. Environ. Microbiol.; (United States)
OSTI ID:6959280
Although xylose is a major product of hydrolysis of lignocellulosic materials, few yeasts are able to convert it to ethanol. In Pachysolen tannophilus, one of the few xylose-fermenting yeasts found, aldose reductase and xylitol dehydrogenase were found to be key enzymes in the metabolic pathway for xylose fermentation. This paper presents a method for the rapid and simultaneous purification of both aldose reductase and xylitol dehydrogenase from P. tannophilus. Preliminary studies indicate that this method may be easily adapted to purify similar enzymes from other xylose-fermenting yeasts.
- Research Organization:
- Dept. of Agriculture, Peoria, IL
- OSTI ID:
- 6959280
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 52:4; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
Similar Records
Temperature sensitivity of the induction of xylose reductase in Pachysolen tannophilus
Xylose fermentation to ethanol by Pachysolen tannophilus
Genetic and biochemical characterization of mutations affecting the ability of the yeast Pachysolen tannophilus to metabolize D-xylose
Journal Article
·
Sat Nov 30 23:00:00 EST 1985
· Biotechnol. Bioeng.; (United States)
·
OSTI ID:6407470
Xylose fermentation to ethanol by Pachysolen tannophilus
Conference
·
Fri Dec 31 23:00:00 EST 1982
· Biotechnol. Bioeng. Symp.; (United States)
·
OSTI ID:6925554
Genetic and biochemical characterization of mutations affecting the ability of the yeast Pachysolen tannophilus to metabolize D-xylose
Journal Article
·
Tue Oct 31 23:00:00 EST 1989
· Applied and Environmental Microbiology; (USA)
·
OSTI ID:5962290
Related Subjects
09 BIOMASS FUELS
090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
550500 -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
BIOCONVERSION
BIOLOGICAL PATHWAYS
CARBOHYDRATES
ENZYMES
FERMENTATION
FUNGI
METABOLISM
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PENTOSES
PLANTS
PURIFICATION
SACCHARIDES
XYLOSE
YEASTS
090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
550500 -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
BIOCONVERSION
BIOLOGICAL PATHWAYS
CARBOHYDRATES
ENZYMES
FERMENTATION
FUNGI
METABOLISM
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PENTOSES
PLANTS
PURIFICATION
SACCHARIDES
XYLOSE
YEASTS