Distinct presynaptic control of dopamine release in striosomal and matrix areas of the cat caudate nucleus

Journal Article · Tue Oct 31 23:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.22.9006· OSTI ID:6957899

Kemel, M L; Desban, M; Glowinski, J; Gauchy, C ^[1]

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1. Institut National de la Sante et de la Recherche Medicale Unite, Paris (France)

By use of a sensitive in vitro microsuperfusion method, the cholinergic presynaptic control of dopamine release was investigated in a prominent striosome (areas poor in acetylcholinesterase activity) located within the core of cat caudate nucleus and also in adjacent matrix area. The spontaneous release of ({sup 3}H)dopamine continuously synthesized from ({sup 3}H)tyrosine in the matrix area was found to be twice that in the striosomal area; the spontaneous and potassium-evoked releases of ({sup 3}H)dopamine were calcium-dependent in both compartments. With 10{sup {minus}6} M tetrodotoxin, 5 {times} 10{sup {minus}5} M acetylcholine stimulated ({sup 3}H)dopamine release in both striosomal and matrix areas, effects completely antagonized by atropine, thus showing the involvement of muscarinic receptors located on dopaminergic nerve terminals. Experiments without tetrodotoxin revealed a more complex regulation of dopamine release in the matrix: (i) in contrast to results seen in the striosome, acetylcholine induced only a transient stimulatory effect on matrix dopamine release. (ii) Although 10{sup {minus}6} M atropine completely abolished the cholinergic stimulatory effect on ({sup 3}H)dopamine release in striosomal area, delayed and prolonged stimulation of ({sup 3}H) dopamine release was seen with atropine in the matrix. The latter effect was completely abolished by the nicotinic antagonist pempidine. Therefore, in the matrix, in addition to its direct (tetrodotoxin-insensitive) facilitatory action on ({sup 3}H)dopamine release, acetylcholine exerts two indirect (tetrodotoxin-sensitive) opposing effects: an inhibition and a stimulation of ({sup 3}H)dopamine release mediated by muscarinic and nicotinic receptors, respectively.

• OSTI ID: 6957899
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:22; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
ALKALI METAL COMPOUNDS
ALKALOIDS
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
ANIMAL TISSUES
ANIMALS
AROMATICS
ATROPINE
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
BRAIN
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CATS
CENTRAL NERVOUS SYSTEM
CHOLINESTERASE
CHROMATOGRAPHY
DOPAMINE
DRUGS
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
HYDROGEN COMPOUNDS
HYDROLASES
HYDROXY ACIDS
HYDROXY COMPOUNDS
ION EXCHANGE CHROMATOGRAPHY
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOLYTICS
PARASYMPATHOMIMETICS
PERFUSED TISSUES
PHENOLS
POLYPHENOLS
POTASSIUM COMPOUNDS
PROTEINS
QUATERNARY COMPOUNDS
RECEPTORS
SECRETION
SEPARATION PROCESSES
SYMPATHOMIMETICS
TISSUES
TRITIUM COMPOUNDS
TYROSINE
VERTEBRATES