The loss of plasma membrane lysopip and an increase of PIP sub 2 result from treatment of carrot cells with fungal enzymes

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6957463

Chen, Q; Boss, W F ^[1]

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1. North Carolina State Univ., Raleigh (USA)

The plasma membranes of carrot cells grown in suspension culture are enriched with PIP, lysoPIP, and PIP{sub 2}. To determine whether or not these lipids are involved in signal transduction, we have challenged the cells with a mixture of fungal cellulases, Driselase, and monitored the changes in the phosphoinositides and in the phosphoinositide kinase activity. With cell prelabeled with ({sup 3}H)inositol, two major changes are observed: (1) lysoPIP decreases 30% compared to the sorbitol control and (2) PIP{sub 2} doubles. There is no increase in IP, IP{sub 2}, or IP{sub 3}. In vitro phosphorylation studies using ({gamma}-{sup 32}P)ATP indicate that the increase in PIP{sub 2} is due in part to activation of the PIP kinase. These data suggest that the role of the polyphosphoinositides in signal transduction in plants may involve activation of the PIP kinase and/or activation of A type phospholipases rather than C type phospholipases.

• OSTI ID: 6957463
• Journal Information: Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 89:4; ISSN PPYSA; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLESTERASES
CARROTS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ENZYMES
ESTERASES
FOOD
HYDROGEN COMPOUNDS
HYDROLASES
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPASES
LIPIDS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MEMBRANES
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PHYSIOLOGY
PLANT CELLS
PLANTS
RADIOISOTOPES
SACCHARIDES
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VEGETABLES