Automating the purification and isolation of synthetic DNA

Journal Article · Thu Jan 01 04:00:00 EST 1987 · Bio/Technology; (United States)

DOI:https://doi.org/10.1038/nbt0187-67· OSTI ID:6956991

Jones, S S; Brown, J E; Vanstone, D A; Stone, D K; Brown, E L

The authors describe the application of robotics to the purification and isolation of synthetic oligodeoxyribunucleotides. Starting with commercially available components, they have designed and programmed a robot based system that can isolate oligonucleotides from polyacrylamide gel slices, prepare oligonucleotides for purification by polyacrylamide gel electrophoresis and execute a rapid purification protocol on a disposable C-18 reverse phase column. Each operation has been fully automated, including evaporative centrifugation, such that the system can execute any two procedures simultaneously with a throughput of 12 samples in about six hours.

• Research Organization: Genetics Institute, Inc., Cambridge, MA (USA)
• OSTI ID: 6956991
• Journal Information: Bio/Technology; (United States), Journal Name: Bio/Technology; (United States) Vol. 5; ISSN BTCHD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CENTRIFUGATION
DNA
ELECTROPHORESIS
FRACTIONATION
NUCLEIC ACIDS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ROBOTS
SEPARATION PROCESSES
ULTRACENTRIFUGATION
USES