Anaerobiosis and ethanol effects on germination, growth, and protein synthesis of five Echinochloa species

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6956571

Dybiec, L D ^[1]; Rumpho, M E; Kennedy, R A ^[2]

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1. Ohio State Univ., Columbus (USA)
2. Univ. of Maryland, College Park (USA)

Five Echinochloa species, encompassing a spectrum from flood tolerant to flood intolerant, were studied to determine the mechanisms of anaerobic germination and growth. Seeds were germinated in air or N{sub 2}, plus 0, 1 or 3% ethanol, and germination rates and growth measurements recorded for 7 days. In air or N{sub 2} increasing ethanol levels did not affect total germination per se, although the rate of germination was delayed in N{sub 2}. Shoot/root lengths in air were highest for tolerant species and increased with increasing ethanol, whereas, in intolerant species, shoot/root lengths decreased with increasing ethanol. Aerobic vs. anaerobic polypeptide profiles of each of the species were compared by SDS/PAGE. For all species, the number of polypeptides decreased under anaerobiosis and several quantitative differences were apparent relative to the aerobic profile. In addition, amino acid incorporation into protein was analyzed by ({sup 35}S)-Met labeling of 3 day old seedlings grown in air or N{sub 2}. Significant protein synthesis was measured in tolerant seedlings under N{sub 2} and several polypeptides were specifically induced. These results are being compared with labeling patterns of the other semi-tolerant and intolerant Echinochloa species to determine their importance in flooding tolerance.

• OSTI ID: 6956571
• Journal Information: Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 89:4; ISSN PPYSA; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

551001 -- Physiological Systems-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
AMINO ACIDS
ANOXIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DRUGS
ETHANOL
EVEN-ODD NUCLEI
GERMINATION
GROWTH
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
METHIONINE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
PLANT GROWTH
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
SEEDS
SULFUR 35
SULFUR ISOTOPES
TOXICITY
TRACER TECHNIQUES