Separation of gonadotropic fractions with different species specificities from tuna pituitaries
Eight different gonadotropic glycoprotein fractions were separated from the acetone-dried powder of yellow fin tuna pituitary glands by successive chromatographies on Superose 12 for gel filtration and Mono Q for anion exchange using the Pharmacia fast protein liquid chromatography system. This was preceded by preliminary separations using an ammonium sulfate precipitation method and affinity chromatography on concanavalin A-Sepharose. For biological characterization, we employed two radioreceptor assay systems, one using goby testis plasma membranes and silver carp GTH as the receptor and radioligand, respectively, and the other using testis plasma membranes of the yellow fin tuna and gonadotropin of the same species, respectively. We also employed two testicular cyclic AMP accumulation bioassay methods in vitro, one with the goby testis and the other with the mackerel testis. The least acidic fraction after Mono Q was further separated into four subfractions by rechromatography with Mono Q. They were strongly active in the tuna and mackerel assays but almost inactive in the goby assays. They were referred to as tuna-type tuna gonadotropin. In contrast, the most acidic fraction obtained after the first Mono Q was active in the goby assays but almost inactive in the tuna and mackerel assays. It was referred to as goby-type tuna gonadotropin. The intermediate fractions were active on both assays and are considered to be mixtures of tuna-type and goby-type gonadotropins. The reason for the presence of gonadotropin inactive to homologous species is discussed from the evolutionary viewpoint.
- Research Organization:
- Waseda Univ., Tokyo (Japan)
- OSTI ID:
- 6954429
- Journal Information:
- Gen. Comp. Endocrinol.; (United States), Vol. 70:2
- Country of Publication:
- United States
- Language:
- English
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GONADOTROPINS
FRACTIONATION
AMP
ELECTROPHORESIS
FISHES
ION EXCHANGE CHROMATOGRAPHY
LIQUID COLUMN CHROMATOGRAPHY
PITUITARY GLAND
RADIORECEPTOR ASSAY
RECEPTORS
SPECIFICITY
TESTES
TUNA
ANIMALS
AQUATIC ORGANISMS
BODY
CHROMATOGRAPHY
ENDOCRINE GLANDS
GLANDS
GONADS
HORMONES
ISOTOPE APPLICATIONS
MALE GENITALS
MEMBRANE PROTEINS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques