Intrinsic polymorphism of variable number tandem repeat loci in the human genome

Journal Article · Mon Sep 12 04:00:00 EDT 1988 · Nucleic Acids Research; (UK)

DOI:https://doi.org/10.1093/nar/16.17.8487· OSTI ID:6949161

Ali, S; Wallace, R B ^[1]

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1. Beckman Research Institute of the City of Hope, Duarte, CA (USA)

In the human genome, short tandem repetitive (STR) DNA sequences often show restriction fragment length polymorphisms (RFLPs) due to variation in the number of copies of the repeat unit. For a subset of these sequences known as minisatellites or variable number tandem repeat loci (VNTR), it has been proposed that a homologous core sequence of 10-12 nucleotides is involved in the mechanism(s) generating the polymorphism. In our present study the authors have prepared oligonucleotide probes complementary to one or two repeat units of several VNTR loci. Under stringent hybridization and wash conditions these probes hybridize locus specifically thus allowing the evaluation of the intrinsic polymorphism of individual loci. The results indicate that not all of the loci having STR DNA sequences are polymorphic despite the fact that they share the core sequence. This suggests that more than the DNA sequence of the locus is involved in the mechanism(s) generating the polymorphism.

• OSTI ID: 6949161
• Journal Information: Nucleic Acids Research; (UK), Journal Name: Nucleic Acids Research; (UK) Vol. 16:17; ISSN 0305-1048; ISSN NARHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DNA HYBRIDIZATION
DNA SEQUENCING
ELECTROPHORESIS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
RFLPS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES