Accelerator mass spectrometry in the biomedical sciences: Applications in low-exposure biomedical and environmental dosimetry

Technical Report · Thu May 10 04:00:00 EDT 1990

DOI:https://doi.org/10.2172/6947352· OSTI ID:6947352

Felton, J S; Turteltaub, K W; Vogel, J S; Balhorn, R; Gledhill, B L; Southon, J R; Caffee, M W; Finkel, R C; Nelson, D E; Proctor, I D; Davis, J C

We are utilizing Accelerator Mass Spectrometry (AMS) as a sensitive detector for tracking the disposition of radio-isotope labeled molecules in the biomedical sciences. These applications have shown the effectiveness of AMS as a tool to quantify biologically important molecules at extremely low levels. For example, AMS is being used to determine the amount of carcinogen covalently bound to animal DNA (DNA adduct) at levels relevent to human exposure. Studies have been undertaken in our laboratory utilizing heterocyclic amine food-borne carcinogens and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a potent environmental carcinogen, to study the metabolism of carcinogens at low doses. In addition, AMS is being used to detect the presence of rare proteins (mutant forms of protamine) in human sperm. Problems in the life sciences where detection sensitivity or sample sizes are limitations should also benefit from AMS. Studies are underway to measure the molecular targeting of cancer chemotherapeutics in human tissue and to pursue applications for receptor biology. We are also applying other candidate isotopes, such as {sup 3}H (double labeling with {sup 14}C) and {sup 41}Ca (bone absorption) to problems in biology. The results described here with {sup 14}C-labeled molecules coupled with new isotope applications clearly show AMS technology to be an important new tool for the biomedical sciences community. 29 refs., 5 figs., 2 tabs.

• Research Organization: Lawrence Livermore National Lab., CA (USA)
• Sponsoring Organization: CANSERC; DOE/DP; NCI; NIEHS
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6947352
• Report Number(s): UCRL-JC-103998; ON: DE90012989; CNN: 222Y-01-ES-70158 CA40811
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ACCELERATOR FACILITIES
ADDUCTS
ALKALINE EARTH ISOTOPES
ALUMINIUM 26
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ANIMALS
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BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOASSAY
BIOCHEMISTRY
BIOLOGICAL MATERIALS
CALCIUM 41
CALCIUM ISOTOPES
CARBON 14
CARBON ISOTOPES
CARCINOGENS
CHEMISTRY
CHEMOTHERAPY
CHLORINE 36
CHLORINE ISOTOPES
COAGULANTS
CONTAMINATION
DAYS LIVING RADIOISOTOPES
DETECTION
DIAGNOSTIC TECHNIQUES
DIOXIN
DNA ADDUCTS
DOSIMETRY
DRUGS
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EVEN-EVEN NUCLEI
EVEN-ODD NUCLEI
HEMATOLOGIC AGENTS
HEPARIN ANTAGONISTS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 131
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LIGHT NUCLEI
MAMMALS
MAN
MASS SPECTROMETERS
MATERIALS
MATERIALS HANDLING
MEASURING INSTRUMENTS
MOLECULAR BIOLOGY
NUCLEI
NUCLEOPROTEINS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PRIMATES
PROTAMINES
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THERAPY
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