Substitution of amino acids in helix F of bacteriorhodopsin: Effects on the photochemical cycle
- Boston Univ., MA (USA)
The effects of amino acid substitutions in helix F of bacteriorhodopsin on the photocycle of this light-driven proton pump were studied. The photocycles of Ser-183----Ala and Glu-194----Gln mutants were qualitatively similar to that of wild-type bacteriorhodopsin produced in Escherichia coli and bacteriorhodopsin from Halobacterium halobium. The substitution of a Phe for either Trp-182 or Trp-189 significantly reduced the fraction of photocycling bacteriorhodopsin. The amino acid substitutions Tyr-185----Phe and Ser-193----Ala substantially increased the lifetime of the photocycle without substantially increasing the lifetime of the M photocycle intermediate. Similar results were also obtained with the Pro-186----Gly substitution. In contrast, replacing Pro-186 with the larger residue Leu inhibited the formation of the M photocycle intermediate. These results are consistent with a structural model of the retinal-binding pocket suggested by low-temperature UV/visible and Fourier transform infrared difference spectroscopies that has Trp-182, Tyr-185, Pro-186, and Trp-189 forming part of the binding pocket.
- OSTI ID:
- 6945909
- Journal Information:
- Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 28:26; ISSN 0006-2960; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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Specific amino acid substitutions in bacterioopsin: replacement of a restriction fragment in the structural gene by synthetic DNA fragments containing altered codons
Patent
·
Tue May 12 00:00:00 EDT 1987
·
OSTI ID:6409756
Amino-terminal sequences of the L,M, and H subunits of reaction centers from the photosynthetic bacterium Rhodopseudomonas sphaeroides R-26
Journal Article
·
Thu Dec 31 23:00:00 EST 1981
· Biochemistry; (United States)
·
OSTI ID:6123793
Specific amino acid substitutions in bacterioopsin: replacement of a restriction fragment in the structural gene by synthetic DNA fragments containing altered codons
Journal Article
·
Sat Mar 31 23:00:00 EST 1984
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:5890918
Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALANINES
AMIDES
AMINES
AMINO ACID SEQUENCE
AMINO ACIDS
AROMATICS
AZAARENES
AZOLES
BACTERIA
BARYONS
CARBOXYLIC ACIDS
CHEMISTRY
ELEMENTARY PARTICLES
ESCHERICHIA COLI
FERMIONS
GLUTAMINE
GLYCINE
HADRONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
INDOLES
INFRARED SPECTRA
LEUCINE
MICROORGANISMS
MOLECULAR STRUCTURE
MUTATIONS
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENYLALANINE
PHOTOCHEMISTRY
PHOTOSYNTHETIC BACTERIA
PIGMENTS
PROLINE
PROTEINS
PROTONS
PYRROLES
PYRROLIDINES
RHODOPSIN
SERINE
SPECTRA
TRYPTOPHAN
59 BASIC BIOLOGICAL SCIENCES
ALANINES
AMIDES
AMINES
AMINO ACID SEQUENCE
AMINO ACIDS
AROMATICS
AZAARENES
AZOLES
BACTERIA
BARYONS
CARBOXYLIC ACIDS
CHEMISTRY
ELEMENTARY PARTICLES
ESCHERICHIA COLI
FERMIONS
GLUTAMINE
GLYCINE
HADRONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
INDOLES
INFRARED SPECTRA
LEUCINE
MICROORGANISMS
MOLECULAR STRUCTURE
MUTATIONS
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENYLALANINE
PHOTOCHEMISTRY
PHOTOSYNTHETIC BACTERIA
PIGMENTS
PROLINE
PROTEINS
PROTONS
PYRROLES
PYRROLIDINES
RHODOPSIN
SERINE
SPECTRA
TRYPTOPHAN