Somatostatin modulates cholinergic neurotransmission in canine antral muscle

Journal Article · Mon Feb 01 04:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6945654

Koelbel, C B; van Deventer, G; Khawaja, S; Mogard, M; Walsh, J H; Mayer, E A ^[1]

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1. Wadsworth Hospital, Los Angles, CA (USA) UCLA Medical Center, Torrance, CA (USA)

Somatostatin has been shown to inhibit antral motility in vivo. To examine the effect of somatostatin on cholinergic neurotransmission in the canine antrum, we studied the mechanical response of and the release of ({sup 3}H)acetylcholine from canine longitudinal antral muscle in response to substance P, gastrin 17, and electrical stimulation. In unstimulated tissues, somatostatin had a positive inotropic effect on spontaneous phasic contractions. In tissues stimulated with substance P and gastrin 17, but not with electrical stimulation, somatostatin inhibited the phasic inotropic response dose dependently. This inhibitory effect was abolished by indomethacin. Somatostatin stimulated the release of prostaglandin E{sub 2} radioimmunoreactivity, and prostaglandin E{sub 2} inhibited the release of ({sup 3}H)acetylcholine induced by substance P and electrical stimulation. Somatostatin increased the release of ({sup 3}H)acetylcholine from unstimulated tissues by a tetrodotoxin-sensitive mechanism but inhibited the release induced by substance P and electrical stimulation. These results suggest that somatostatin has a dual modulatory effect on cholinergic neutrotransmission in canine longitudinal antral muscle. This effect is excitatory in unstimulated tissues and inhibitory in stimulated tissues. The inhibitory effect is partially mediated by prostaglandins.

• OSTI ID: 6945654
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 254:2; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
ALKALOIDS
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
ATROPINE
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOELECTRICITY
BIOLOGICAL EFFECTS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DOGS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTRICITY
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
GASTRIN
HORMONES
HYDROGEN COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MUSCLES
NERVE CELLS
NEUROREGULATORS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASYMPATHOLYTICS
PARASYMPATHOMIMETICS
PEPTIDE HORMONES
PEPTIDES
POLYPEPTIDES
PROSTAGLANDINS
PROTEINS
QUATERNARY COMPOUNDS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
SECRETION
SOMATIC CELLS
SOMATOSTATIN
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES