Histone phosphorylation during differentiation of HL-60 cells induced by phorbol diesters
Thesis/Dissertation
·
OSTI ID:6945333
Phorbol ester effects on histone phosphorylation during induction of differentiation were examined in quiescent HL-60 cells. Following exposure to TPA, differentiation was determined by adherent properties, morphological maturity, and nonspecific esterase (NSE) activity. Changes in histone phosphorylation were concurrently investigated in the presence of /sup 32/P-phosphoric acid and histones fractionated using two-dimensional gel electrophoresis. Addition of TPA produced changes in H2B phosphorylation which were observable as early as 30 minutes, peaked at 4 hours, then slowly decreased to control levels.
- Research Organization:
- Cincinnati Univ., OH (USA)
- OSTI ID:
- 6945333
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARCINOGENS
CELL DIFFERENTIATION
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
HISTONES
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEOPROTEINS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PROTEINS
RADIOISOTOPES
TRACER TECHNIQUES
TWO-DIMENSIONAL ELECTROPHORESIS
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARCINOGENS
CELL DIFFERENTIATION
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
HISTONES
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEOPROTEINS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PROTEINS
RADIOISOTOPES
TRACER TECHNIQUES
TWO-DIMENSIONAL ELECTROPHORESIS